Taxonomic Observations of the Isolates of Rhizopus species Inhabiting at the Raw Materials for Korean Traditional Fermented Foods in Korea

한국 전통 발효식품의 원료에서 채집된 Rhizopus속의 분류학적 관찰

  • Lee, Sang-Sun (Department of Biological Science and Education, Graducate School, Korea National University of Education) ;
  • Yoon, Young-Sil (Department of Biological Science and Education, Graducate School, Korea National University of Education) ;
  • Yu, Kee-Won (Department of Food Science and Technology, College of Agriculture, Chung-Nam National University) ;
  • Sung, Chang-Kun (Department of Food Science and Technology, College of Agriculture, Chung-Nam National University)
  • 이상선 (한국교원대학교 대학원, 생물과학 및 생물교육학) ;
  • 윤영실 (한국교원대학교 대학원, 생물과학 및 생물교육학) ;
  • 유기원 (충남대학교 농과대학 식품공학과) ;
  • 성창근 (충남대학교 농과대학 식품공학과)
  • Published : 1998.12.30

Abstract

The isolates of Rhizopus collected from Korean traditional fermented food meju and nuruk, were compared with the well known species of Rhizopus donated. The isolates of Rhizopus were identified with the numerical analyses calculated from RAPD-DNA bands, and confirmed with the microscopic observations of morphological features on PDA. The isolates of R. oryzae purchased were segregated through the results of RAPD or the morphological features. The species of R. nigricans, known as an illegitimate species, were different from those of R. stolonife and it is estimated that they are species of Rhizopus, again. According to microscopic observations and the result of RAPD, Some isolates of R. oryzae purchased belong to R. nigricans and are completely different from R. oryzae in their growth. The isolates of R. nigricans included with several isolates of R. oryzae identified for the different phenotypes and were heterogenous. The isolate of R. oligosporus were speculated to be different from morphological features of Rhizopus, but to be similar to the species of Absidia on the apophysis of sporangium. Its sporangiophore or mycelium was observed to be dark black, but the sporangia were not in those of R. oligosporus. The isolates collected from Korean traditional nuruk showed genetic diversity, and also considered to be different tastes in Korean rice wines.

Keywords

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