Formation and Characteristics of Oidia in Flammulina velutipes

팽이버섯 분열자의 생성원인 및 특성

  • Kim, Young-Ho (Division of Applied Microbiology, National Inst. of Agri. Sci. & Tech., RDA) ;
  • Kong, Won-Sik (Division of Applied Microbiology, National Inst. of Agri. Sci. & Tech., RDA) ;
  • Kim, Kyung-Soo (Division of Applied Microbiology, National Inst. of Agri. Sci. & Tech., RDA) ;
  • You, Chang-Hyun (Division of Applied Microbiology, National Inst. of Agri. Sci. & Tech., RDA) ;
  • Kim, Han-Kyoung (Division of Applied Microbiology, National Inst. of Agri. Sci. & Tech., RDA) ;
  • Sung, Jae-Mo (Department of Agricultural Biology, Kangwon National Univ.) ;
  • Ryu, Young-Jin (Department of Agricultural Biology, Kangwon National Univ.) ;
  • Kim, Kwang-Ho (Department of Agronomy, Kon-Kuk Univ.)
  • 김영호 (농업과학기술원 응용미생물과) ;
  • 공원식 (농업과학기술원 응용미생물과) ;
  • 김경수 (농업과학기술원 응용미생물과) ;
  • 유창현 (농업과학기술원 응용미생물과) ;
  • 김한경 (농업과학기술원 응용미생물과) ;
  • 성재모 (강원대 농생물학과) ;
  • 류영진 (강원대 농생물학과) ;
  • 김광호 (건국대 농학과)
  • Published : 1998.06.30

Abstract

The effects of medium, incubation temperature, incubation period, pH of medium and $CO_2$ ondition during mycelial growth were investigated to study the factors associated with the formation of oidia in Flammulina velutipes. Oidia formation was increased when mycelial growth was poor, while oidia formation was inhibited in optimum condition of mycelial growth. Mating type of oidia was investigated to examine the effect of oidia formation on dikaryotic strain. Di-mon matings between oidia strains and original dikaryotic strain were carried out. Monokaryotic strains derived from oidia showed only one genotype. Seventy percent among Dimon mating strains showed slow mycelial growth and low yield of fruit-body, but others showed similar or high mycelial growth and yields in comparision with original dikaryon strain. One strain from di-mon mating demonstrated some differences in isozyme band pattern.

팽이버섯균주의 분열자의 생성원인을 구명하기 위하여 균사생장시의 배지종류, 배양온도, 배양기간, pH, 및 $CO_2$ 농도별로 균사생장정도와 분열자 생성수를 조사하여 본 결과, 저온시 균사의 분화가 제대로 일어나지 않아 균사가 완전히 생장하지 못했던 경우를 제외하고는 균사생장이 불량한 조건에서 분열자의 생성은 많았으며, 최적조건에서는 분열자의 생성량이 적었다. 이핵균주로부터 유래된 분열자를 발아시켜 얻은 단핵균주의 교배형은 모두 한 가지의 교배형의 핵을 가졌으며, 모균주에 미치는 영향을 조사하기 위하여 19개의 분열자균주를 모균주와 Di-Mon교배를 실시한 결과 모균주에 비해 교배균주의 70%는 공시균주에 비하여 균사생장이 늦고 수량이 적었으며 Di-Mon 교배균주 중 한 균주는 동위효소 pattern에서도 차이를 나타내었다.

Keywords

References

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