Bipolaris coicis의 완전세대인 Cochliobolus nisikadoi의 배양기내 형성

In vitro Formation of Cochliobolus nisikadoi, the Perfect State of Bipolaris coicis

  • 김성기 (경기도 농촌진흥원 식물환경과) ;
  • 김기우 (서울대학교 농업생명과학대학 응용생물.화학부) ;
  • 박은우 (서울대학교 농업생명과학대학 응용생물.화학부) ;
  • 강위수 (서울대학교 농업생명과학대학 응용생물.화학부) ;
  • 양장석 (경기도 농촌진흥원 식물환경과)
  • Kim, Sung-Kee (Department of Plant and Environment, Kyonggi Provincial Rural Development Administration) ;
  • Kim, Ki-Woo (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Seoul National University) ;
  • Park, Eun-Woo (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kang, Wee-Soo (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Seoul National University) ;
  • Yang, Jang-Souck (Department of Plant and Environment, Kyonggi Provincial Rural Development Administration)
  • 발행 : 1998.06.30

초록

율무 잎마름병을 일으키는 Bipolaris coicis의 완전세대를 배양기내에서 형성하도록 유도하였다. 국내 17개 지역에서 1994년부터 1996년까지 89개의 균주를 수집하였다. 이들을 $25^{\circ}C$에서 볏짚을 위에 올려놓은 Sach's nutrient agar 배지에서 대치배양하였다. 위자낭각은 특정 친화적인 균주의 조합일 때만 형성되었다. 위자낭각은 대체로 배양 2주 후에 볏짚 위에 형성되었으나, 일부 위자낭각에서만 자낭과 자낭포자가 관찰되었다. 위자낭각은 흑색이며 구형이었고, 돌출한 ostiolar beak을 가지고 있었다. 그 내부는 무색이고, 사상형민 위측사로 채워져 있었다. 자낭은 원통형내지 곤봉형이었고, 직선형이거나 약간 굽은 것도 있었다. 자낭벽은 이중격막으로 둘러싸여 있었고 짧은 stipe가 있었다. 자낭포자는 사상형이고 무색이었으며, 자낭의 장축과 평행하거나 약간 나선형으로 굽어 있었다. 자낭포자의 크기는 $146-166.4{\times}2.6-3.8\;{\mu}m$이었다. 이러한 형태적 특징에 기초하여 이 곰팡이의 완전세대는 Cochliobolus nisikadoi (Tsuda, Ueyama & Nishihara Alcorn)으로 동정되었다.

The perfect state of Bipolaris coicis, causing leaf blight of Job's tears, was in duced under in vitro conditions. Eighty nine isolates of the fungus were collected from 17 locations in Korea from 1994 to 1996. They were crossed on Sach's nutrient agar, on which a piece of rice straw was placed, and incubated at $25^{\circ}C$. Pseudothecia were produced only by certain combinations of compatible isolates. Although pseudothecia were usually produced on rice straw two weeks after incubation, asci and ascospores were observed only in a few pseudothecia examined. The pseudothecia were black and globose with protruding ostiolar beaks. The locules were filled with a mass of hyaline and filamentous pseudoparaphyses. Asci were cylindrical to clavate and straight or slightly curved. The ascus wall was bitunicate with short stipes. Ascospores were filiform, hyaline, and arranged parallel to slightly coiled in the asci, measuring $143-166.4{\times}2.6-3.8\;{\mu}m$. Perfect state of the fungus was identified as Cochliobolus nisikadoi (Tsuda, Ueyama & Nishihara Alcorn), based on the morphological characteristics.

키워드

참고문헌

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