The Korean Journal of Physiology and Pharmacology
- 제2권1호
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- Pages.119-131
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- 1998
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
The Inhibitory Mechanism of Aloe Glycoprotein (NY945) on the Mediator Release in the Guinea Pig Lung Mast Cell Activated with Antigen-Antibody Complexes
- Ro, Jai-Youl (Departments of Pharmacology, Yonsei University College of Medicine) ;
- Lee, Byung-Chul (Departments of Molecular Biology, Korea university College of Life Science) ;
- Chung, Myung-Hee (Department of Pharmacology, Seoul National University College of Medicine) ;
- Lee, Seung-Ki (Department of Biochemistry, Seoul National University College of Pharmacy) ;
- Sung, Chung-Ki (Department of Pharmaceutics, Chunnam University College of Pharmacy) ;
- Kim, Kyung-Hwan (Departments of Pharmacology, Yonsei University College of Medicine) ;
- Park, Young-In (Departments of Molecular Biology, Korea university College of Life Science)
- 발행 : 1998.02.21
초록
It has been reported that the glycoprotein extracted from Aloe has strong anti-inflammatory response. However, there has been no research report yet about the effect of Aloe on allergic hypersensitivity reactivity. By using guinea pig lung mast cells, this study aimed to examine the effects of Aloe glycoprotein (NY945) on the mediator releases caused by mast cell activation, and also aimed to assess the effects of NY945 on the mechanism of mediator releases in the mast cell activation. We partially purified mast cell from guinea pig lung tissues by using the enzyme digestion, the rough and the discontinuous density percoll gradient method. Mast cells were sensitized with IgG1 (anti-OA) and challenged with ovalbumin. Histamine was assayed by fluorometric analyzer, leukotrienes by radioimmunoassay. The phospholipase D activity was assessed by the production of labeled phosphatidylalcohol. The amount of mass 1, 2-diacylglycerol (DAG) was measured by the
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