The Korean Journal of Physiology and Pharmacology
- Volume 2 Issue 1
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- Pages.95-100
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- 1998
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
The Characterization of the Increase of Membrane Conductance after Depolarization in Single Rat Adrenal Chromaffin Cells
- Lim, Won-Il (Department of Physiology & Biophysics, Seoul National University College of Medicine) ;
- Kim, Sang-Jeong (Department of Physiology & Biophysics, Seoul National University College of Medicine) ;
- Kim, Jun
- Published : 1998.02.21
Abstract
The conductance change evoked by step depolarization was studied in primarily cultured rat adrenal chromaffin cells using patch-clamp and capacitance measurement techniques. When we applied a depolarizing pulse to a chromaffin cell, the inward calcium current was followed by an outward current and depolarization-induced exocytosis was accompanied by an increase in conductance trace. The slow inward tail current which has the same time course as the conductance change was observed in current recording. The activation of slow tail current was calcium-dependent. Reversal potentials agreed with Nernst equation assuming relative permeability of
Keywords
- Chromaffin cell;
- Small conductance calcium-activated potassium channel;
- Patch-clamp techniques;
- Membrane conductance;
- Membrane capacitance