The Journal of Natural Sciences (자연과학논문집)
- Volume 9 Issue 1
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- Pages.25-29
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- 1997
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- 1225-6196(pISSN)
Isolation of the Gene for Lipocortin-1 Binding Protein Using Yeast Two Hybrid Assay
Yeast Two Hybrid Assay를 이용한 Lipocortin-1 결합 단백질 유전자의 분리
- Lee, Koung-Hoa (Department of Biochemistry, Pai-Chai University) ;
- Kim, Jung-Woo (Department of Biochemistry, Pai-Chai University)
- Published : 1997.02.28
Abstract
To study the mechanism of lipocortin-1, the 37 kDa protein, one of the annxin superfamily thought to be a second messenger during the Glucocorticoid dependent anti-inflammatory action, the gene for lipocortin-1 binding protein was isolated using the yeast two hybrid assay, the yeast based genetic assay recognizing the protein-protein interaction. The results showed that this gene has a weak homology to the for the human serine proteinase.
Glucocorticoid에 의한 항염증 작용의 second messenger로 생각되어지는 annexin superfamily중 하나인 37 kDa의 단백질, lipocortin-1의 작용기작을 이해할 목적으로 in vivo에서 protein-protein interaction을 인식하는 yeast-based genetic assay인 yeast two assay를 통하여 lipocortin-1과 결합하는 단백질 유전자를 분리하여 조사하였다. 이 방법으로 실험을 수행한 결과 분리된 유전자가 human serine proteinase 유전자와 homology가 있는 것으로 밝혀졌다.