Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
권호 표지

Cryopreservation of Testicular Spermatozoa using Mouse Zona Pellucida in Intracytoplasmic Sperm Injection Program

ICSI 프로그램에서 생쥐 투명대를 이용한 고환조직내 정자의 동결

  • Suh, Tae-Kwang (Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital) ;
  • Jeon, Byeong-Gyun (Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital) ;
  • Ryu, Eun-Kyung (Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital) ;
  • Lee, Eun-Sook (Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital) ;
  • Ryoo, Zae-Yoong (Catholic Research Institute of Medical Science, College of Medicine, Catholic University) ;
  • Sohn, Sea-Hwan (Department of Dairy Science & Technology, Chinju National University) ;
  • Moon, Jin-Soo (Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital) ;
  • Kim, Kwang-Chull (Institute of Infertility and Cytogenetics, Kaya Mother-Child's Hospital)
  • 서태광 (가야자모병원 불임 유전연구소) ;
  • 전병균 (가야자모병원 불임 유전연구소) ;
  • 류은경 (가야자모병원 불임 유전연구소) ;
  • 이은숙 (가야자모병원 불임 유전연구소) ;
  • 류재웅 (카톨릭의과대학교 의과학연구원) ;
  • 손시환 (진주산업대학교 낙농자원학과) ;
  • 문진수 (가야자모병원 불임 유전연구소) ;
  • 김광철 (가야자모병원 불임 유전연구소)
  • Published : 1997.08.30
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Abstract

The survival rate and motility recovered after cryopreservation of testicular spermatozoa in testicular sperm extraction (TESE)-ICSI program is low. The purpose of this study was to assess the availability and efficiency of mouse empty zona pellucida in cryopreserving human TESE spermatozoa. Mouse empty zonae pellucidae were obtained by extraction of cytoplasm with or without cytochalasin B treatment. Motile sperm from proven-fertile donor and two azoospermic patients after TESE were individually inserted into empty zona pellucida and cryopreserved. Two to five days after cyropreservation, the frozen sperm were thawed and the rates of recovery and motility were observed. The ooplasmic extraction rates of control (N=80) and cytochalasin B treated oocytes (N=80) were 94.0% and 96.2%, respectively (p>0.05). The post-thaw recovery rates of spermatozoa and rates of motility recovery of ejaculate (N=70) and testicular (N=70) sperm were 97.1%, 97.1% and 95.7%, 94.3%, respectively (p>0.05). The results of this study showed that the mouse zone pellucida is useful for cryostorage of single testicular spermatozoa.

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