Clinical and Experimental Reproductive Medicine
- 제24권3호
- /
- Pages.377-383
- /
- 1997
- /
- 2233-8233(pISSN)
- /
- 2233-8241(eISSN)
배아밀도와 배양액 용량이 착상전후의 생쥐배아의 체외 성장에 미치는 영향
Effect of Embryo Number and Incubation Volume on the Development of Pre- and Post-implantation Mouse Embryos In Vitro
- 강병문 (울산대학교 의과대학 산부인과학교실) ;
- 전용필 (울산대학교 의과대학 산부인과학교실) ;
- 김지영 (울산대학교 의과대학 산부인과학교실) ;
- 김정희 (울산대학교 의과대학 산부인과학교실) ;
- 이지윤 (울산대학교 의과대학 산부인과학교실) ;
- 채희동 (울산대학교 의과대학 산부인과학교실) ;
- 김정훈 (울산대학교 의과대학 산부인과학교실) ;
- 장윤석 (울산대학교 의과대학 산부인과학교실) ;
- 목정은 (울산대학교 의과대학 산부인과학교실)
- Kang, Byung-Moon (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan) ;
- Cheon, Yong-Pil (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan) ;
- Kim, Ji-Young (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan) ;
- Kim, Jeong-Hee (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan) ;
- Lee, Ji-Yun (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan) ;
- Chae, Hee-Dong (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan) ;
- Kim, Chung-Hoon (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan) ;
- Chang, Yoon-Seok (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan) ;
- Mok, Jung-Eun (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan)
- 발행 : 1997.12.30
초록
The effects of embryo number and incubation volume on the development of mouse embryos were evaluated. The growth rate of two-cell mouse embryos to attached blastocyst stage and the growth rate of blastocysts to early somite stage were assessed after culture in different incubation volumes and embryo densities. Embryos were collected from ICR female mice superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin and mated by ICR males. In experiment 1, groups of one, five, ten, twenty 2-cell embryos were cultured in 10-, 50-, 500-, 1000-