Tuberculosis and Respiratory Diseases
- Volume 44 Issue 3
- /
- Pages.470-478
- /
- 1997
- /
- 1738-3536(pISSN)
- /
- 2005-6184(eISSN)
Phagocytosis of Drug-Resistant Mycobacterium Tuberculosis by Peripheral Blood Monocytes
결핵균의 약제내성과 말초혈액단핵구의 결핵균 탐식능에 관한 연구
- Park, Jae-Seuk ;
- Kim, Jae-Yeal (Department of Internal Medicine and Tuberculosis Research Institute, Seoul National University, College of Medicine) ;
- Yoo, Chul-Gyu (Department of Internal Medicine and Tuberculosis Research Institute, Seoul National University, College of Medicine) ;
- Kim, Young-Whan (Department of Internal Medicine and Tuberculosis Research Institute, Seoul National University, College of Medicine) ;
- Han, Sung-Koo (Department of Internal Medicine and Tuberculosis Research Institute, Seoul National University, College of Medicine) ;
- Shim, Young-Soo (Department of Internal Medicine and Tuberculosis Research Institute, Seoul National University, College of Medicine)
- 박재석 (단국대학교 의과대학 내과학교실) ;
- 김재열 (서울대학교 의과대학 내과학교실 및 결핵연구소) ;
- 유철규 (서울대학교 의과대학 내과학교실 및 결핵연구소) ;
- 김영환 (서울대학교 의과대학 내과학교실 및 결핵연구소) ;
- 한성구 (서울대학교 의과대학 내과학교실 및 결핵연구소) ;
- 심영수 (서울대학교 의과대학 내과학교실 및 결핵연구소)
- Published : 1997.06.30
Abstract
Background : Phagocytosis is probably the first step for mycobacteria to be virulent in host because virulent strains are more readily phagocytosed by macrophage than attenuated strains. According to the traditional concept, multi-drug resistant strains have been regarded as less virulent. However, this concept has been challenged, since recent studies(reported) showed that the degree of virulence and drug-resistance is not related. The purpose of this study is to evaluate whether the phagocytic activity of M.tuberculosis by peripheral blood mononuclear cells(PBMC) is different according to drug-resistance or host factor. To evaluate this, we estimated the difference of phagocytic activity of drug-resistant and drug-sensitive M.tuberculosis and also estimated the phagocytic activity of PBMC from intractable tuberculosis patients and healthy controls. Methods : PBMC from ten intractable tuberculosis patients and twelve healthy control, and three different strains of heat-killed M.tuberculosis, ie, ADS(all drug sensitive), MDR(multi-drug resistant), and ADR(all drug resistant) were used. After incubation of various strains of M.tuberculosis with PBMC, the phagocytic activity was evaluated by estimating proportion of PBMC which have phagocytosed M.tuberculosis. Results : Drug-resistant strains of M.tuberculosis were phagocytosed easily than drug sensitive strains(Percentage of PBMC phagocytosed M.tuberculosis in healthy control : ADS :
연구배경 : 세포내 병원균(intracellular pathogen)으로 감염초기에 단핵식세포(mononuclear phagocytes)에 탐식되는데, 독성(virulent) 결핵균주(Erdman, H37Rv)가 약독화(attenuated) 균주(H37Ra)보다 탐식이 더 잘 되며, 이러한 현상으로부터 결핵균의 입장에서 보면 단핵식세포에 탐식이 잘되는 것이 생존 및 증식에 유리할 것이라는 의견이 제시되어 왔다. 그리고 난치성 결핵환자에서 분리한 결핵균은 임상적으로 독성이 강하며 대부분에서 다제내성을 보이므로, 약제내성 결핵균여 감수성균에 비해 독성이 강하다는 가정하에 약제내성 결핵균이 감수성균에 비해 단핵식세포에 탐식이 더 잘 되는지, 그리고 난치성 결핵환자에서 분리한 단핵식세포가 정상인에 비해 결핵균을 더 잘 탐식하는지 알아보고자 하였다. 방 법 : 약제내성의 정도에 따라 모든약제에 감수성인 결핵균, Isoniazid와 Rifampicin을 포함한 일부 약제에 내성인 결핵균, 모든 약제에 내성인 결핵균을 각각
Keywords
- Multi-drug resistant Mycobacterium tuberculosis;
- Intractable tuberculosis patient;
- Phagocytosis of Mycobacterium tuberculosis