농촌의학ㆍ지역보건 (Journal of agricultural medicine and community health)
- 제22권1호
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- Pages.61-74
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- 1997
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- 1738-9577(pISSN)
조직기생 선충류 유충에서 분리한 단백 분해 효소의 특성 및 항원성 검토
Determination of Antigenicity and Characterization of Proteinase from Tissue Invading Nematode Larvae
- 임한종 (고려대학교 의과대학 기생충학교실 및 열대풍토병연구소) ;
- 주경환 (고려대학교 의과대학 기생충학교실 및 열대풍토병연구소) ;
- 최성아 (고려대학교 의과대학 기생충학교실 및 열대풍토병연구소) ;
- 이혜정 (고려대학교 의과대학 기생충학교실 및 열대풍토병연구소) ;
- 주종윤 (계명대학교 의과대학 기생충학교실) ;
- 정명숙 (계명대학교 의과대학 기생충학교실)
- Rim, Han-Jong (Department of Parasitology, College of Medicine and Institute for Tropical Endemic Diseases, Korea University) ;
- Joo, Kyeong-Hwan (Department of Parasitology, College of Medicine and Institute for Tropical Endemic Diseases, Korea University) ;
- Choi, Sung-A (Department of Parasitology, College of Medicine and Institute for Tropical Endemic Diseases, Korea University) ;
- Lee, Hye-Jeong (Department of Parasitology, College of Medicine and Institute for Tropical Endemic Diseases, Korea University) ;
- Joo, Chong-Yoon (Department of Parasitology, School of Medicine Kemyung University) ;
- Chung, Myung-Sook (Department of Parasitology, School of Medicine Kemyung University)
- 발행 : 1997.06.30
초록
In case of tissue invading nematode, proteolytic enzyme was required at their parasitic life. Proteinases obtained from these parasites(Toxocara canis, Ansakis spp. and Trichinella spiralis) were extracted, isolated and further purified. And then the analysis for activity and inhibitory effect of proteinases were performed by appropriate substrate. Determination of protein as a circulating antigen was done in use of infected animal serum with above parasites, respectively. For above experimental objects, following procedures were performed. First, enzymatic activity was measured in use of azocasein and inhibitory effect of porteinase were studied by various inhibitors. Second, partially purified proteins containing enzymatic activity were obtained by ion exchange chromatography, ultrafiltration and electrophoretic elution. Third, role of the partially purified protein as a circulating antigen. The results obtained were as follows : 1. Enzymatic activity of each nematode proteinase was varied according to pH. Optimal pH of Toxocara canis, Ansakis spp. and Trichinella spiralis were pH 6.0, pH 5.5 and pH 6.5, respectively. The optimal molarity of buffer was 0.1M phosphate buffer. Although little difference between these proteinases was observed, temperature stability was at least maintained at
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