Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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CYTOTOXICITY OF DENTIN BONDING SYSTEM ON L929 CELLS

상아질 결합제의 L929 세포 독성에 관한 연구

  • Im, Mi-Kyung (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Ji, Jung-Ho (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Kim, Sang-Seop (Department of Conservative Dentistry, College of Dentistry, Wonkwang University)
  • 임미경 (원광대학교 치과대학 치과보존학교실) ;
  • 지정호 (원광대학교 치과대학 치과보존학교실) ;
  • 김상섭 (원광대학교 치과대학 치과보존학교실)
  • Published : 1997.04.08
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Abstract

The research of the dentin bonding system was mainly on the chemistry and bonding strength. And in vitro assessement of biocompatibility of dentin bonding system was not completely developed. The purpose of this study was to evaluate the cytotoxic effect of several dentin primers. Scotchbond Multi-Purpose (3M Dental Products. USA). Gluma (BayerDental. Germany). All-Bond (Bisco. USA). ProBond (CaulkDensply, USA) and VeridonFil (Dongyang Nylon. Korea) were included. Cytotoxicity was tested using MTT cell viability test. 0.5 ul. 1 ul. 2 ul and 10 ul of each primer were added to the 96 well plate of incubated L929 cell lines. After 30-minute. 1. 4. 24 and 72-hour exposures. absorbance of L929 cells was observed with ELISA reader. All data were analyzed using t-test. All primers showed cytotoxicity on L929 cells under every conditions used in this study. Absorbance of L929 cells was decreased by time. Scotch bond group exhibited the lowest absorbance value in all exposure time and value.

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Acknowledgement

Supported by : 원광대학교