Restorative Dentistry and Endodontics
- 제22권1호
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- Pages.76-92
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- 1997
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- 2234-7658(pISSN)
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- 2234-7666(eISSN)
Porphyromonas endodontalis와 Porphyromonas gingivalis의 단백질분해능력에 관한 연구
COMPARISON OF PROTEOLYTIC ACTIVITY OF PORPHYROMONAS ENDODONTALIS AND PORPHYROMONAS GINGIVALIS
- Ha, Joo-Hee (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University) ;
- Choi, Ho-Young (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University)
- 발행 : 1997.04.08
초록
Porpilyromonas endodontalis is specifically involved in endodontic infections. The bacterium can be isolated almost exclusively only from infected rool canals. P. gingivalis also has been implicated in endodontic infection. Pathogemcity of P. gingival is is attributed to a variety of virulence factors, especially proteases, produced by the bacterium. Importance of P. endodontalis in endodontic infection has been revealed. However, the pathogenic property of P. endodontalis has not been extensively studied. The present study was undertaken to characterize the proteolytic activity of P. endodontalis and compare the activity with that of P. gingivalis which has the most potent and diverse proteases among oral bacteria. For this purpose, culture supematants(SUP) and cell extracts(CE) were obtained from these two bacteria and were subjected to zymography using 15% polyacrylamide gel copolymerized with gelatin, type I, IV collagens or albumin. Hydrolysis of the collagens was further investigated by the cleavage assay using native type I and IV collagens in solution-phase. The results were as follows: 1. P. endodontalis apparently has a proteolytic activity that is comparable with that of P. gingivalis. 2. SUP and CE obtained from P. endodontalis and P. gingival is showed the strongest activity for gelatin, followed by type I and IV collagens, and albumin. 3. In the zymography, no noticeable difference in proteolytic activity for gelatin and albumin between the SUP and CE was observed, but in the cleavage assay using native collagens, the SUP showed a stronger collagenolytic activity than the CE. 4. The gelatinolytic activity of both the SUP and CE from these two bacteria was diminished in the presence of
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