대한세포병리학회지 (The Korean Journal of Cytopathology)
- 제8권1호
- /
- Pages.27-34
- /
- 1997
- /
- 1017-0391(pISSN)
기관지 폐포 세정액에서 뉴우모시스티스 카리니의 면역세포화학적 검출
Immunocytochemical Detection of Pneumocystis carinii in Bronchoalveolar Lavage
- 권건영 (계명대학교 의과대학 병리학교실 및 의과학연구소) ;
- 조승제 (계명대학교 의과대학 병리학교실 및 의과학연구소) ;
- 김상표 (계명대학교 의과대학 병리학교실 및 의과학연구소) ;
- 박관규 (계명대학교 의과대학 병리학교실 및 의과학연구소) ;
- 장은숙 (계명대학교 의과대학 병리학교실 및 의과학연구소) ;
- 김정숙 (영남대학교 의과대학 임상병리학교실)
- Kwon, Kun-Young (Department of Pathology and Institute of Medical Science, Keimyung University School of Medicine) ;
- Cho, Seung-Che (Department of Pathology and Institute of Medical Science, Keimyung University School of Medicine) ;
- Kim, Sang-Pyo (Department of Pathology and Institute of Medical Science, Keimyung University School of Medicine) ;
- Park, Kwan-Kyu (Department of Pathology and Institute of Medical Science, Keimyung University School of Medicine) ;
- Chang, Eun-Sook (Department of Pathology and Institute of Medical Science, Keimyung University School of Medicine) ;
- Kim, Chung-Sook (Department of Clinical Pathology, Yeongnam University School of Medicine)
- 발행 : 1997.06.30
초록
Pneumocystis carinli is an established cause of pulmonary infections in immuno-compromised hosts. Several cytoiogical stains, such as Papanicolaou, Gomori methenamine sliver(GMS) and Diff-Quik have been used for detection of the organism, but occasionally can be laborious and, due to a degree of nonspecificity, may be misleading. We evaluated the diagnostic utility of immunocytochenmical stains that recognize P. carinii in bornchoalveolar lavage from experimentally Induced P. carinii pneumonia rats(n=15). In audition to routine stains for diagnosis by morphologic recognition of P. carinii on Papanicolaou, GMS and Diff-Quik stains, bronchoalveolar lavage samples were reacted with immunocytochemical stains using monoclonal antibodies(MAB) 092 and 902. In bronchoalveolar lavage P. carinii organisms were detected In 9 of 10 cases(90%) using each MAB 092 and 902, whereas GMS and Diff-Quik stains demonstrated P. carinii in 13(86%) and 11(73%) of 15 cases respectively. In lung tissue specimens(n=15) P. carinii organisms were well identified on GMS stain and immunohistochemical stains using MAB 092 and 902 in ail cases. We believe that the immunocytochemical staining using MAB 092 and/or 902 is a very useful and diagnostic tool In addition to GMS and Diff-Qulk stain to detect P. carinii organisms in bronchoalveolar lavage.
키워드