대한수의학회지 (Korean Journal of Veterinary Research)
- 제37권4호
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- Pages.875-882
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- 1997
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Staphylococcus aureus의 재조합 fibronectin-binding protein의 생산
Production of the recombinant fibronectin-binding protein of Staphylococcus aureus
-
kim, Doo
(Department of Veterinary Medicine, Kangwon National University) ;
- Cheong, Cha-ryong (Department of Veterinary Medicine, Kangwon National University) ;
-
Park, Hee-myong
(College of Veterinary Medicine, Seoul National University) ;
- Han, Hong-ryul (College of Veterinary Medicine, Seoul National University)
- 투고 : 1997.08.07
- 발행 : 1997.12.25
초록
To produce the recombinant fibronectin-binding protein(FnBP) for development of subunit vaccine against Staphylococcus aureus. The fnbp gene was amplified from the chromosomal DNA of S aureus KNU 196 strain using the polymerase chain reaction, and cloned into pGEX-4T-2. Then, the recombinant FnBP fused with glutathione-S-transferase was produced in E coli, purified by affinity chromatography, and identified its antigenicity and immunogenicity by Western blot. The recombinant FnBP produced in this study is considered to have the same property of native FnBP purified from S aureus, and is expected to be useful as a candidate for S aureus subunit vaccine.