Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Production of the recombinant fibronectin-binding protein of Staphylococcus aureus

Staphylococcus aureus의 재조합 fibronectin-binding protein의 생산

  • kim, Doo (Department of Veterinary Medicine, Kangwon National University) ;
  • Cheong, Cha-ryong (Department of Veterinary Medicine, Kangwon National University) ;
  • Park, Hee-myong (College of Veterinary Medicine, Seoul National University) ;
  • Han, Hong-ryul (College of Veterinary Medicine, Seoul National University)
  • 김두 (강원대학교 축산대학 수의학과) ;
  • 정자룡 (강원대학교 축산대학 수의학과) ;
  • 박희명 (서울대학교 수의과대학) ;
  • 한홍율 (서울대학교 수의과대학)
  • Received : 1997.08.07
  • Published : 1997.12.25
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Abstract

To produce the recombinant fibronectin-binding protein(FnBP) for development of subunit vaccine against Staphylococcus aureus. The fnbp gene was amplified from the chromosomal DNA of S aureus KNU 196 strain using the polymerase chain reaction, and cloned into pGEX-4T-2. Then, the recombinant FnBP fused with glutathione-S-transferase was produced in E coli, purified by affinity chromatography, and identified its antigenicity and immunogenicity by Western blot. The recombinant FnBP produced in this study is considered to have the same property of native FnBP purified from S aureus, and is expected to be useful as a candidate for S aureus subunit vaccine.

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