Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Discrimination of Two Red Algae Acrosorium polyneurum and A. yendoi Using Polymerase Chain Reaction Technique

유전자증폭반응 기법을 이용한 홍조류 잔금분홍잎 및 누은분홍잎의 구별

  • KIM Long-Guo (Dept. of Biotechnology, Pukyong National University) ;
  • JIN Hyung-Joo (Dept. of Biotechnology, Pukyong National University) ;
  • KIM Young-Sik (Dept. of Marine Biology, Pukyong National University) ;
  • PARK Jung-Youn (Biotechnology Division, Aquaculture Department, National Fisheries Research and Development Agency) ;
  • NAM Ki-Wan (Dept. of Marine Biology, Pukyong National University) ;
  • HONG Yong-Ki (Dept. of Biotechnology, Pukyong National University)
  • Published : 1997.07.01
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Abstract

The polymerase chain reaction (PCR) technique was used to distinguish from two morphologically similar red algal species; Acrosorium polyneurum and A. yendoi. Total DNA was extracted by the LiCl method. The extracted DNA (15 ng) in a $25{\mu}\ell$ reaction volume was amplified by the PCR technique using primers covering with mitochondrial D-loop gene, nuclear rDNA internal transcribed spacer (ITS), and nuclear rDNA external transcribed spacer. A. yendoi could be distinguished from A. polyneurum on the producible basis of amplified ITS fragment of 650 bp.

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