Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Chemometric Aspects and Determination of Sugar Composition of Honey by HPLC

HPLC에 의한 꿀 중의 당조성 분석과 화학계량학적 고찰

  • Yoon, Jung-Hyeon (Advanced Analysis Center SIMS, AES/SAM, KIST) ;
  • Bae, Sun-Young (Department of Chemistry, University of Massachusetts) ;
  • Kim, Kun (Department of Chemistry, Seoul Women's University) ;
  • Lee, Dong-Sun (Department of Chemistry, Seoul Women's University)
  • 윤정현 (한국과학기술연구원 특성분석센터) ;
  • 배선영 (매사츄세츠대학교 화학과) ;
  • 김건 (서울여자대학교 자연과학대학 화학과) ;
  • 이동선 (서울여자대학교 자연과학대학 화학과)
  • Received : 1997.05.30
  • Published : 1997.10.25
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Abstract

Chemometric technique was applied to the sugar composition in five honeys of known botanical or geographical origin following HPLC. Fructose and glucose were predominant carbohydrates in honeys, and small amount of sucrose was also detected in one sample. Sugar contents in honeys samples were compared by the geographical or botanical origin. Fructose/glucose ratio ranged from 0.99 to 1.55 was obtained and these results are in good agreement with the ratio of literature. The plot of principal components analysis(PCA) showed that different honey samples grouped into distinct cluster by the geographical or botanical origin. Increasing the first or second principal component score, higher amount of sugar or less fructose/glucose ratio was observed in PCA plot. Chemometric approach was very useful to provide pattern recognition of sugar profile or quality indices of honey sample and to detect adulteration.

HPLC를 이용하여 생산지와 밀원꽃이 알려진 5종의 꿀 중 당성분을 분석한 다음 화학계량학적 고찰을 수행하였다. 꿀의 주된 당성분은 fructose, glucose였으며, 1종의 꿀에서는 sucrose가 소량 검출되었다. 산지별, 밀원꽃별로 당 함량을 비교하였다. Fructose/glucose 함량비는 0.99~1.55 범위로서 문헌값과 잘 일치하였다. Principal components analysis(PCA) plot은 산지별, 밀원꽃별로 확연히 구분되었다. 주성분점수가 커질수록 당 함량이 증가되었고 fructose/glucose 함량비는 감소되었다. 화학계량학적 접근방법은 꿀시료의 당조성 패턴을 비교하고 품질평가 및 부정화 적발에 유용하였다.

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