Applied Microscopy
- Volume 27 Issue 3
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- Pages.333-346
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- 1997
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- 2287-5123(pISSN)
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- 2287-4445(eISSN)
Effects of DMTU, SOD and Ischemic Preconditioning on the Ultrastructural Changes of the Rectus Femoris Muscles in Rats after Ischemia and Reperfusion
SOD, DMTU및 허혈양상화 처치가 허혈 및 재관류에 의한 흰쥐 넙다리곧은근의 미세구조 변화에 미치는 영향
- Paik, Doo-Jin (Department of Anatomy, College of Medicine, Hanyang University) ;
- Lim, Jae-Hyun (Department of Anatomy, College of Medicine, Hanyang University) ;
- Chung, Ho-Sam (Department of Anatomy, College of Medicine, Hanyang University)
- Published : 1997.09.01
Abstract
The ischemia and reperfusion injury of the skeletal muscles is caused by generation of reactive oxygen during ischemia and reperfusion. It is well known that over 4 hours of ischemia injures the skeletal muscles irreversibly. The author has demonstrated the effects of SOD (superoxide dismutase), DMTU (dimethyl thiourea) and ischemic preconditioning on ultrastructural changes of the muscle fibers in the rectus femoris muscles after 4 hours of ischemia and 1 day and 3 days of reperfusion. A total of 72 healthy Sprague-Dawley rats weighing from 200 gm to 250 gm were used as experimental animals. Under urethane(1.15 g/kg, IP, 2 times) anesthesia, lower abdominal incision was done and the left common iliac artery was occluded by using vascular clamp for 4 hours. The left rectus femoris muscles were obtained at 1 and 3 days after the removal of vascular clamp. The SOD (15,000 unit/kg) or DMTU (500 mg/kg) were administered intraperitoneally at 1 hour before induction of ischemia. The ischemic preconditioned group underwent three episodes of 5 minutes occlusion and 5 minutes reperfusion followed by 4 hours of ischemia and 1 day and 3 days of reperfusion. The specimens were sliced into