치과방사선 (Journal of Korean Academy of Oral and Maxillofacial Radiology)
- 제27권2호
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- Pages.91-103
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- 1997
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- 1225-049X(pISSN)
시험관내 A-253 세포주의 방사선 및 항암제 감수성에 관한 연구
A STUDY ON THE RADIOSENSITIVITY AND CHEMOSENSITIVITY OF A-253 CELL LINE IN VITRO
- Lee Joo-Hyun (Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University) ;
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Hwang Eui-Hwan
(Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University) ;
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Lee Sang-Rae
(Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University)
- 발행 : 1997.08.01
초록
The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy. For this study, cell surviving curves were obtained for human epidermoid carcinoma A-253 cell line using semiautomated MTT assay. 2,4,6,8,10 Gy were irradiated at a dose rate of 210 cGy/min using /sup 60/Co Irradiator ALDORADO 8. After irradiation, A-253 cell lines(2×10⁴cells/mil were exposed to bleomycin or cisplatin for 1 hour. The viable cells were determined for each radiation dose with/without 2 /lg/mi of drug at the 3rd day. And they were compared to control values. The results were obtained as follows : 1. The surviving curve with gentle slope was obtained after irradiation of 2, 4, 6, 8, 10 Gy on A-253 cell line. 2. The cytotoxicity of bleomycin or cisplatin at the concentration of 2㎍/ml was great on A-253 cell line. But, there was no significant difference between the cytotoxicity of bleomycin and that of cisplatin. 3. There were significant differences of surviving fractions after irradiation with 2㎍/mi of bleomycin compared with irradiation only on A-253 cell line. 4. There were significant differences of surviving fractions after irradiation with 2㎍/ml of cisplatin compared with irradiation only on A-253 cell line. 5. There were no significant differences of surviving fractions between the groups of irradiation with bleomycin and the groups of irradiation with cisplatin on A-253 cell line.
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