Journal of Korean Society of Forest Science (한국산림과학회지)
- Volume 86 Issue 3
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- Pages.261-269
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- 1997
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- 2586-6613(pISSN)
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- 2586-6621(eISSN)
Transient Expression of β-gulucuronidase (GUS) gene in Immature Ovules and Calli Derived from Cottonwood Species (Populus deltoides) by Microprojectile Bombardment
포플러의 미성숙(未成熟) 배(胚)와 캘러스에서 유전자총(遺傳子銃)에 의(依)한 GUS-gene의 일시적(一時的) 발현(發現)
- Kang, Hoduck (Forestry Administration, Forest Environment Division) ;
- Kang, Sang-Gu (Department of Zoology and Genetics, College of Liberal Arts and Sciences, Iowa State University) ;
- Bae, Hanhong (Department of Forestry, College of Agriculture, Iowa State University) ;
- Park, Kyo-Soo (Dongguk University, College of Life Resources, Department of Forest Resources) ;
- Hall, Richard B. (Department of Forestry, College of Agriculture, Iowa State University)
- Received : 1996.05.23
- Published : 1997.09.30
Abstract
Excised immature ovules and calli derived from the stems of cottonwood were bombarded with microprojectiles carrying plasmid DNA containing CaMV-35S promoter and
미류나무의 미성숙 ovule과 줄기로부터 유기된 캘러스에 plasmid pBI221 유전자를 유전자총을 이용하여 인위적으로 삽입하였다. Plasmid pBI221은 CaMV-35S 유전자에 의하여 발현되는
Keywords
- Particle bombardment;
- transient gene expression;
- GUS gene expression;
- Populus;
- immature ovule;
- calli