YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Pharmacokinetics of Recombinant Human Epidermal Growth Factor (DWP401) in Rats

재조합 인간 상피세포성장인자(DWP401)의 흰쥐에서의 약물동태

  • 정주영 ((주)대웅제약 중앙연구소) ;
  • 고여욱 ((주)대웅제약 중앙연구소) ;
  • 남권호 ((주)대웅제약 중앙연구소) ;
  • 조재열 ((주)대웅제약 중앙연구소) ;
  • 박승국 ((주)대웅제약 중앙연구소) ;
  • 유영효 ((주)대웅제약 중앙연구소) ;
  • 김재환 ((주)대웅제약 중앙연구소) ;
  • 한건 (충북대학교 약학대학) ;
  • 박명환 ((주)대웅제약 중앙연구소) ;
  • 심창구 (서울대학교 약학대학) ;
  • Published : 1997.06.01
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Abstract

Pharmacokinetics of DWP401, a recombinant human epidermal growth factor (rhEGF), was studied using radioimmunoassay (RIA) and $^{125}I$-DWP401 in rats. When DWP401 was adm inistered i.v. at doses of 50 and 500 mcg/kg, the plasma DWP401 disappeared biiexponentially with terminal half life of 4.7 and 92.8 min. The $C_{max}$ and $T_{max}$ after s.c. administration of ti at doses of 50 and 500 ${\mu}g$/kg were determined to be 23.6 and 17.5 ng/ml at 50 ${\mu}g$/kg, and 261.4 ng/ml and 36.8 min, respectively. Both the total urinary and biliary recoveries of intact DWP401 2343 very low (<0.4%), probably due to its extensive degradation in the body. the concentration ratio of DWP401 between the organ and plasma decreased especially in the liver and kidney as the dose and time after the dose increased. For example, the liver/plasma and kidney/plasma concentration ratio of DWP401 at 2.5 min after i.v. doses of 50 ${\mu}g$/kg were comparable and much larger than unity. But, the ratio at 2.5 min after i.v. doses of 500${\mu}g$/kg was much larger in the kidney that in than in the liver. These results suggest that the systemic administration of DWP401 might be subject to rapid and extensive clearance from circulation within several hour after main distrbution to liver and kidney.

Keywords

References

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