약학회지 (YAKHAK HOEJI)

  • 제41권1호
  • /
  • Pages.99-104
  • /
  • 1997
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

산소자유기에 의해 저해된 배양 척수감각 신경절 세포에 대한 상피세포성장인자의 영향

Effect of EGF against Oxygen Radical-Induced Neurotoxicity in Cultured Spinal Dorsal Root Ganglion Neurons of Mouse

  • 박승택 (원광대학교 의과대학 해부학교실) ;
  • 김형룡 (원광대학교 치과대학 치과약리학교실) ;
  • 채한정 (원광대학교 약학대학)
  • Park, Seung-Taeck (Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Kim, Hyung-Ryong (Department of Dental Pharmacology, and School of Dentistry, Wonkwang University) ;
  • Chae, Han-Jung (College of Pharmacy, Wonkwang University)
  • 발행 : 1997.02.01
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초록

In order to elucidate the cytotoxic effect of oxygen radicals on cultured spinal dorsal root ganglion(DRG) neurons derived from mouse. the neurotoxic effect of oxygen radicals w as examined after cultured DRG neurons were exposed to xanthine oxidase(XO) and hypoxanthine(HX)-oxygen radical generating system. In addition. neuroprotective effect of epidermal growth factor(EGF) against oxidant-induced neurotoxicity was also evaluated in these cultures. The results were, as follows: 1. Lethal concentration 50(LC$_{50}$) was 35mU/ml XO and 0.1mM HX in cultured DRG neurons. 2. Oxygen radicals induced the morphological changes such as the decrease of cell number and loss of neurites in these cultures. 3. EGF increased the cell viability and neurofilament in neurons damaged by oxygen radicals. From above the results, it is suggested that oxygen radicals have a cytotoxic effect on cultured DRG neurons of neonatal mouse and selective neurotrophic factors such as EGF are, effective, in blocking the neurotoxicity induced by oxygen radicals in cultured spinal DRG neurons.

키워드

참고문헌

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