The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 27 Issue 1
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- Pages.59-68
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- 1997
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- 1225-2344(pISSN)
Molecular Characterization of a New Hantaan Virus Howang Strain
한탄바이러스 호왕균주의 M, S 유전자 절편의 염기서열 및 분자생물학적 특성
- Chu, Yong-Kyu (Asan Institute for Life Sciences) ;
- Lee, Ho-Wang (Asan Institute for Life Sciences)
- Published : 1997.06.30
Abstract
Hantaan virus Howang strain which isolated from the blood of severe case of Korean hemorrhagic fever is more virulent than HTN 76/118 and showed different RFLP from partial PCR amplifed M genome segment to established Hantaan serotype viruses. We have determined the nucleotide sequence of the M and S genome segments and compared to HTN 76/118. The M and S segment of Howang strain has 3615 and 1696 nucleotides long, respectively. The M segment sequence of Howang strain is one mucleotide shorter than HTN 76/118. The sequence data of Howang strain shows 93.5% homology to HTN 76/118. One long open reading frame, which strats from 41nt. to 3448nt. of the M segment and from 37nt. to 1326nt. of the S segment, exist to on complementary sense of the virus genome. There are no significant difference between HTN 76/118 and Howang strain on hydrophobicity of deduced polypeptides, but has slight difference on secondary structure.