대한바이러스학회지 (The Journal of Korean Society of Virology)
- 제27권2호
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- Pages.169-176
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- 1997
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- 1225-2344(pISSN)
Enterovirus에 대한 분자생물학적 검증법 및 Genotypes 방법의 개발
The Development of Molecular Detection Method and Differentiation of Genotypes of Enterovirus
- 김은순 (울산대학교 의과대학 미생물학교실) ;
- 남정현 (울산대학교 의과대학 미생물학교실) ;
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김기순
(국립보건원 바이러스질환부 소화기계바이러스과) ;
- 윤재득 (국립보건원 바이러스질환부 소화기계바이러스과) ;
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김유겸
(울산대학교 의과대학 미생물학교실)
- Kim, Eun-Soon (Department of Microbiology, College of Medicine, Univ. of Ulsan) ;
- Nam, Jung-Hyun (Department of Microbiology, College of Medicine, Univ. of Ulsan) ;
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Kim, Ki-Soon
(Division of Enteroviruses, Department of Virology, National Institute of Health) ;
- Yoon, Jae-Deuk (Division of Enteroviruses, Department of Virology, National Institute of Health) ;
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Kim, Yoo-Kyum
(Department of Microbiology, College of Medicine, Univ. of Ulsan)
- 발행 : 1997.12.30
초록
In this study, the feasibility of identification and genotypic differentiation of enteroviruses was investigated by using nested reverse transcription-polymerase chain reaction (nested RT-PCR), single-stranded conformation polymorphism (SSCP), and restriction fragment length polymorphism (RFLP) techniques. Two hundred seventy-four clinical samples were assayed by both nested RT-PCR and tube culture method using MRC-5 and MK cells; 58 (86.6%) out of 67 enterovirus culture-positive samples contained enteroviral RNA. In addition, 114 (55.1%) of 207 samples from patients with suspected enteroviral CNS disease with negative viral cultures were positive by the nested RT-PCR. The nested RT-PCR products were genotyped by the SSCP method and the results were compared with serotypes. We could differentiate 6 subtypes, 3 of which are similar to coxsackievirus B3, B5, echovirus 11, plus 3 other subtypes. RFLP cleaved with Sty I, Bgl I, and Xmn I yielded characteristic patterns for each laboratory strains. This study demonstrates the usefulness of the RT-PCR for the rapid diagnosis of enterovirus infection and the potentials of the SSCP method for differentiation of enterovirus strains.