대한바이러스학회지 (The Journal of Korean Society of Virology)
- 제27권2호
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- Pages.143-149
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- 1997
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- 1225-2344(pISSN)
당첨가가 B형 간염 바이러스 백신의 안정성에 미치는 영향
Effects of Addition of Sugars on the Stability of Hepatitis B Virus Vaccine
- 성인화 (고려대학교 의과대학 미생물학교실, 고려대학교 부설 바이러스병 연구소)
- Seong, In-Wha (Department of Microbiology, Medical College, Korea University, Institute for Viral Diseases, Korea University)
- 발행 : 1997.12.30
초록
Most of the current licenced hepatitis B vaccines are being produced by recombinant DNA technology in large fermentation cultures of Saccharomyces cerevisiae of yeast cells which carry the gene coded for hepatitis B virus surface antigen. These vaccines are proved very effective clinically and the immunogenicity of vaccines could be maintained for a long time under refrigeration. To develope the stabilizer that could increase the stability of hepatitis B virus vaccine which could be stored for a long period at room temperature or higher conditions, glucose, lactose and sucrose solutions in phosphate buffered saline were added into hepatitis B vaccine respectively to make 2.5%, 5%, 7.5% and 10% final concentration in vaccines. These sugar-vaccine mixtures were stored at room temperature for one month, two months and three months respectively and then inoculated into ICR mice intramuscularly. On the fourteenth day after inoculation, mice were bled and sera were tested for the evaluation of efficacies of vaccines. The results showed that 5% glucose, 7.5% lactose and sucrose increased the stability of vaccines in some degree and this method could be applied for the production of other viral vaccines and bacterial vaccines.