The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 27 Issue 2
- /
- Pages.137-141
- /
- 1997
- /
- 1225-2344(pISSN)
The Evaluation of Immunochromatographic Assay kit for Rapid Detection of Hepatitis B Surface Antigen
Hepatitis B Surface Antigen을 신속히 검출하기 위한 Immunochromatographic Assay kit의 성능 평가
- Shin, Hyeong-Soon (Chong Kun Dang Research Institute) ;
-
Kim, Young-Bong
(Chong Kun Dang Research Institute)
;
- Shin, Jung-Woo (Chong Kun Dang Research Institute) ;
- Kim, Chang-Kyu (Chong Kun Dang Research Institute) ;
- Lee, Wang-Sik (Chong Kun Dang Research Institute) ;
-
Kim, Han-Kyeom
(Department. of Pathology, Medical College, Korea University)
;
- Shin, Kwang-Soon (Department. of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University)
- 신형순 (주식회사 종근당 종합연구소) ;
-
김영봉
(주식회사 종근당 종합연구소)
;
- 신정우 (주식회사 종근당 종합연구소) ;
- 김창규 (주식회사 종근당 종합연구소) ;
- 이왕식 (주식회사 종근당 종합연구소) ;
-
김한겸
(고려대학교 의과대학 병리학과)
;
- 신광순 (충남대학교 수의과대학 수의학과)
- Published : 1997.12.30
Abstract
We evaluated Immunochromatographic assay kit to screen HBsAg in human serum. When the reference HBsAg was applyed to ICA, HA and EIA kits, the limit of detection for HBsAg were found out to be 4, 2 and 0.25 ng/ml respectively. But ICA kit required 5 minutes to read the result whereas HA and EIA kit more than one hour. The sensitivity was 97% (29 of 30 samples) and the specificity 100% (45 samples) compared with conventional EIA. The ICA kit needs no instrument or machine to perform the test contrary to the conventional methods. Therefore, this rapid and sensitive ICA kit can be used for HBsAg-screening, especially in the emergency room and in the scene of the accident.