Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
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Urinary Profiling of Endogeneous Estrogens Using GC/MS

GC/MS에 의한 뇨 중 내인성 에스트로겐의 Profiling

  • Lee, Seon Hwa (Doping Control Center, Korea Institute of Science and Technology) ;
  • Yang, Yoon Jung (Doping Control Center, Korea Institute of Science and Technology) ;
  • Kim, Tae Wook (Ansung NAtional University, Department of Environmental Engineering) ;
  • Paeng, Ki Jung (Yonsei University, Department of Chemistry) ;
  • Chung, Bong Chul (Doping Control Center, Korea Institute of Science and Technology)
  • 이선화 (한국과학기술연구원 도핑콘트롤센터) ;
  • 양윤정 (한국과학기술연구원 도핑콘트롤센터) ;
  • 김태욱 (국립안성산업대학교 환경공학과) ;
  • 팽기정 (연세대학교 문리대학 화학과) ;
  • 정봉철 (한국과학기술연구원 도핑콘트롤센터)
  • Published : 19970400
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Abstract

Estrogens, which play an important role in sex hormone and potential inhibitor of cancer cell proliferation were profiled for normal female and male. The nineteen endogeneous estrogens were simultaneously analyzed by selected ion monitoring method of GC/MS. Urinary estrogens were extracted by using Serdolit AD-2 resin, hydrolyzed with $\beta-glucuronidase/arylsulfatase$ from Helix pomatia, liquid-liquid extraction and quantitatively derivatized by MSTFA/TMSCl mixture in order to be detected on the GC/MS. The good quality-control data were obtained through the precision and accuracy test and the recovery range of them was 80.97∼97.81%. The Korean reference values of urinary estrogens were established and differences were found in normal female compared with normal male.

성 호르몬으로서의 역할과 암 성장의 잠재적 저해 효과를 지니고 있는 에스트로겐의 대사체들을 동시에 분석하기 위하여 Serdolit AD-2 수지와 효소 가수분해, 액체-액체 추출방법을 이용한 전처리 과정을 확립하였으며, MSTFA/TMSCl 혼합액에 의해 trimethylsilylether 형태로 유도체화된 에스트로겐들을 GC/MS의 SIM(selected ion monitorning) 방법으로 동시에 분석, 정량할 수 있는 조건을 설정하였다. 그 결과 회수율은 80.97~97.81%이었고, within-a-day 및 day-to-day 분석에서의 RSD값은 각각 0.24~25.35%, 1.05~23.94%이었으며, 이 방법으로는 소변 내에 존재하는 19종의 에스트로겐 대사체들을 정량하여 한국인 정상인 참고치(여자:22~35세, 남자: 39~57세)를 설정하였다.

Keywords

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