Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

A Structure-Based Activation Model of Phenol-Receptor Protein Interactions

  • Published : 1997.01.20
Download PDF
⟨ Previous Next ⟩

Abstract

Data from structure/activity studies in vir gene induction system have led to evaluate the working hypothesis of interaction between phenolic inducers and phenol binding proteins. The primary specificity in the association of a phenolic inducer with its receptor in our system is hypothesized to be the hydrogen bonding interactions through the ortho methoxy substituents as well as the proton transfer between the inducer and the binding protein. In this paper the proposed working model for phenol-mediating signal transduction was evaluated in several ways. The importance of the general acid-base catalysis was first addressed by the presence of an acidic residue and a basic residue in the phenol binding protein. Series of compounds were tested for vir gene expression activity to confirm the generation of a strong nucleophile by an acidic residue and an involvement of a basic residue as a proton acceptor. An attempt was made to correlate the pKa values of the phenolic compounds with vir gene induction activities as inducers to further support the proposed proton transfer mechanism. Finally, it was also observed that the regioselectively attached methoxy group on phenol compounds is required as the proper hydrogen bond acceptor.

Keywords

References

  1. Annu. Rev. Microbiol. v.42 Binns, A. N.;Thomashaw, M. F.
  2. Annu. Rev. Biochem. v.60 Bourret, R. B.;Borkovich, K. A.;Simon, M. I.
  3. Proc. Natl. Acad. Sci. USA v.89 Lee, K.;Dudley, M. W.;Hess, K. M.;Lynn, D. G.;Joerger, R. D.;Binns, A. N.
  4. Science v.232 Boulton, G. W.;Nester, E. W.;Gordon, M. P.
  5. Phytochemistry v.27 Spencer, P. A.;Towers, G. H. N.
  6. J. Mol. Microbiol. v.3 Melchers, L. S.;Regensburg-Tuink, A. J. G.;Schilperoot, R. A.;Hooykaas, P. J.
  7. J. Mol. Biol. v.208 Schreuder, H. A.;Prick, P. A. J.;Wierenga, R. K.;Vriend, G.;Wilson, K. S.;Hol, W. G. J.;Drenth, J.
  8. Proc. Natl. Acad. Sci. USA v.88 Hess, K. M.;Dudley, M.W.;Lynn, D. G.;Joerger, R. D.;Binns, A. N.
  9. Biochem. Biophys. Res. Comm. v.33 Hartman, F. C.
  10. Chem. Rev. v.69 Benson, S. W.;Cruickshank, F. R.;Golden, D. M.;Haugen, G. R.;O'Neal, H. E.;Rogers, A. S.;Shaw, R.;Walsh, R.
  11. Ph.D. Thesis, The University of Chicago Initiation of signal transduction pathways by phenolics in plants and bacteria Hess, K. M.
  12. pKa prediction for organic acids and bases Perrin, D. D.;Boyd, D.;Sergeant, E. P.
  13. Pure & Appl. Chem. v.65 Lee, K.;Tzeng, Y.-L.;Liu, N.;Dudley, M. W.;Lynn, D. G.
  14. Phytochemistry v.27 Spencer, P. A.;Tower, G. H. N.
  15. Ph.D. Thesis, The University of Chicago Synthetic probes of signal transduction pathways Dudley, M.
  16. Ph.D. Thesis, The University of Chicago Cyclopropylquinone: A probe of redox processes in development and a substrate for a unique photoannulation reaction Zeng, Z.
  17. Proc. Natl. Acad. Sci. USA v.71 Chilton, M. D.;Currier, T.;Farrand, S.;Bendich, A.;Gordon, M.;Nester, E. W.
  18. EMBO J. v.5 Stachel, S. E.;Nester, E. W.