KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)
- Volume 42 Issue 6
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- Pages.790-799
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- 1997
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- 0252-9777(pISSN)
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- 2287-8432(eISSN)
Application of AFLPs to Phylogenetic Analysis of Aegilops
AFLPs에 의한 Aegilops의 계통발생학적 재평가
- Park, Yong-Jin (National Institute of Agricultural Science & Technology) ;
- Shim, Jae-Wook (Coll. of Agri. & Life Sci., Seoul Nat'l Unv.)
- Published : 1997.12.01
Abstract
Aegilops genus is known to include the donor species of the Band D genome of the bread wheat(ABD). An effort to establish a better strategy for phylogenetic relationships about Aegilops polyploids by AFLPs(Amplified Fragment Length Polymorphisms) was conducted using the 19 Aegilops sPP. and T. aestivum. The 207 polymorphic bands from the amplified products on the 6% acrylamide denaturing sequencing gels were obtained with the 7 AFLP primer combinations, and used to account for the genetic similarities and cluster analysis using NTSYS program. According to the genome analysis, the
각 게놈형간의 근연관계 및 배수체종의 게놈분석에 관한 새로운 접근을 시도하고자, Aegilops 19종 및 재배밀(T. aestivum)을 공시하여 AFLP 분석을 실시하여 얻은 결과는 다음과 같다. 1. AFLPs를 이용한 Aegilops종들간 근연관계를 분석한 결과, 7개의 primer 조합에서 총 207개의 다형 band를 조사하였으며 조합당 평균 다형 band수는 29.8개 이었다. 2. 각 게놈간 유연관계로 보아 Ae. heldreichii (