Journal of Microbiology and Biotechnology
- 제7권3호
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- Pages.161-166
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- 1997
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Molecular Characterization of Intergeneric Hybrids between Trichoderma harzianum and Gliocladium virens
- Shin, Pyung-Gyun (Horticulture Division, National Alpine Agricultural Experiment Station, RDA) ;
- Ryu, Jin-Chang (Molecular Genetics Division, Agricultural Science and Technology Institute, RDA) ;
- Yoo, Young-Bok (Molecular Genetics Division, Agricultural Science and Technology Institute, RDA) ;
- Jeong, Won-Hwa (Boryung Biopharma Co., LTD) ;
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Cho, Moo-Je
(Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
- 발행 : 1997.06.01
초록
Nuclei were isolated from the protoplasts of Trichoderma harzianum T95 and treated with colchicine, a polyploid inducer. The nuclei were transferred into the protoplast of multi-auxotrophic Gliocladium virens G88 which cannot grow in minimal medium. The protoplast of G. virens G88 carrying the transferred nuclei were regenerated in a regeneration minimal medium containing