감자 바이러스 Y 저항성 형질전환 감자 개발

Development of Potato Virus Y-Resistant Transgenic Potato

  • PARK, Young Doo (Department of Horticulture, Kyung-Hee University) ;
  • RONIS D.H. (Department of Plant Sciences, North Dakota State University) ;
  • DUYSEN M.E. (Department of Plant Sciences, North Dakota State University) ;
  • CHENG Z.M. (Department of Plant Sciences, North Dakota State University) ;
  • LORENZEN J.H. (Department of Plant Sciences, North Dakota State University)
  • 발행 : 1997.09.01

초록

ND860-2, Norchip, Russet Norkotah, Goldrush 및 Norgueen Russet 등 5개 품종의 감자 잎 조직을 감자 바이러스 Y(PVY)의 외피단백질 (CP) 유전자를 운반하는 Agrobacterium tumefaciens을 이용하여 형질전환시켰다. ND860-2와 Norchip품종과 같은 흰색 감자에서 재분화율과 형질전환율이 높게 나타났다. 형질전환체를 선발한 후 CP의 생성을 western blot으로 확인한 결과 30 kD의 밴드가 나타났다. 온실에서 재배된 형질전환체는 한 개체를 제외하고는 표현형에 있어서는 기존의 품종과 다르지 않았다. 인위적으로 감자 바이러스 Y를 감염시킨 15일 후의 검사결과 control구는 80%의 감염율을 보인 반면 형질전환체들은 38-58%의 감염율을 보였다. 30일 후의 검사 결과는 모든 5품종의 control구에서 100%의 감염을 보였으나 형질전환체는 7개체가 저항성을 보였다. 마지막 85일 후의 검사 결과 4개의 저항개체를 발견하였으며 이 개체들은 현재 계속 연구가 진행 중에 있다.

Leaf segments of the potato (Solanum tuberosum L.) genotypes, ND860-2, Norchip, Russet Norkotah, Goldrush, and Norqueen Russet were transformed with the coat protein gene of potato virus Y (PVY). The white-skinned genotypes, ND860-2 and Norchip, were easily transformed and regenerated into shoots, whereas the three russet-skinned genotypes had low frequencies of regeneration. Transformed shoots were generally recovered in four to six weeks. Antibody to PVY coat protein detected a single band of 30 kD in western blots of transgenic plants. Transformed plants had a normal phenotype in the greenhouse and many showed a delayed buildup of PVY following inoculation. Several transgenic lines had negative ELISA readings 85 days after inoculation. Transgenic lines which did not show detectable levels of PVY antigen will be further tested for resistance to PVY.

키워드

참고문헌

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