Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Transformation of Brassica napus via Agrobacterium Vector : Plant Regeneration and Progeny Analysis

Agrobacterium 운반체를 이용한 유채의 형질전환: 식물체재분화와 후대검정

  • KIM, Kyung Min (Department of Agronomy, College of Agriculture, Kyungpook National University) ;
  • SOHN, Jae Keun (Department of Agronomy, College of Agriculture, Kyungpook National University) ;
  • CHUNG, Jae Dong (Department of Horticulture, College of Agriculture, Kyungpook National University)
  • Published : 1997.09.01
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Abstract

Cotyledonary petioles of Brassica napus cocultivated with Agrobacterium vectors for 72 h were transferred to MS medium with 0.5 mg/L NAA, 2.0 mg/L BA, 30 mg/L kanamycin, 100 mg/L cefotaxime, 30 g/L sucrose, 3 mg/L $\textrm{AgNO}_{3}$ and 2 g/L Gelrite. The cotyledonary petioles with green shoots were selected at a frequency of 17.5% in a selection medium and then rooted. Southern blot analysis confirmed the rolC and NPT IIgenes were incorporated into the regenerated plants. The stable inheritance of rolC gene was confirmed in progeny test of transgenic plants.

유채의 자엽조직을 rolC 유전자를 가진 Agrobacterium 운반체와 공동배양하여 형질전환체를 얻고, 후대에 대한 유전분석을 실시하였다. Arobacterium 운반체와 공동배양된 유채의 자엽조직을 0.5mg/L NAA, 2.0mg/L BA, 30mg/L kanamycin, 100mg/L cefotaxime, 30mg/L sucrose, 3mg/L $\textrm{AgNO}_{3}$ 및 2 g/L Gelrite가 첨가된 MS 배지에 배양하였을 때 형질전환된 식물체의 분화율이 17.5%로 나타났다. 형질전환체로 확인된 식물체를 자가수정시켜 얻어진 후대에 대한 유전분석을 실시한 바 rolC 유전자가 후대로 유전됨을 확인 할 수 있었다.

Keywords

References

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