Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Genetic Transformation of Intact Potato Microtuber by Particle Bombardment

Particle Bombardment 방법을 이용한 인공 씨감자의 형질전환

  • 최경화 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.) ;
  • 전재흥 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.) ;
  • 김현순 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.) ;
  • 정영희 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.) ;
  • 임용표 (충남대학교 농과대학 원예학과) ;
  • 정혁 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.)
  • Published : 1997.03.01
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Abstract

In vitro grown microtubers of potato (cv Jaju) were used for introduction of herbicide resistance gene using bombardment with DNA-coated particles. The apical shoot-tip area of newly sprouted microtubers were intensively bombarded. After bombardment, microtubers were germinated and transplanted in a greenhouse. Northern blot analysis indicated that bar gene was expressed in two plantlets. After 5 weeks of growing, commercial herbicide Basta was sprayed to screen the resistant plants. All untransformed potato plants died after 7 days while two transgenic plants survived.

제초제 저항성 유전자를 코팅시킨 입자를 particle bombardment 기법으로 자주감자 품종의 인공씨감자에 도입시켰다. 발아직전에 있는 인공씨감자의 생장점 부위를 집중적으로 particle bombardment 한 후 발아한 인공씨감자를 온실에 심었다. 온실에서 5주동안 생육시킨 후 northern 분석결과 유전자가 발현된 2개의 형질전환체를 선발하였고 제초제 살포 실험을 한 결과 형질전환 되지않은 식물체는 모두 고사하였지만 형질전환된 2개의 자주감자 식물체는 생존하였다.

Keywords

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