Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of Several Additives on Plant Regeneration from Leaf Disc Culture of Solanum tuberosum L.

감자의 잎 절편 배양시 몇 가지 배지의 첨가물이 식물체 재분화에 미치는 영향

  • 최경화 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.) ;
  • 전재흥 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.) ;
  • 김현순 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.) ;
  • 정영희 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.) ;
  • 정혁 (한국과학기술연구원 생명공학연구소 식물조직배양 R.U.)
  • Published : 1997.01.01
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Abstract

The effects of several additives on plant regeneration were investigated from leaf disc culture of S. tuberosum cv. Atlantic which is known as poor in regeneration ability. The presence of 2 g/L casein hydrolysate significantly enhanced shoot regeneration. Addition of 10~20 $\mu$M $AgNO_3$, not only increased the frequency of shoot regeneration but also maintained the leaf disc green presumably by the inhibitory action of ethylene accumulation in vitro. Decrease of sucrose levels to below 3% significantly increased the degree of regeneration. The addition of CuSO$_4$had no effect on shoot regeneration.

몇 가지 종류의 배지첨가물이 재분화가 어려운 것으로 알려져 있는 감자 애틀란틱품종의 잎 절편 배양시 shoot 형성에 미치는 영향을 조사하였다. 카세인 가수분해물은 2 g/L 농도가 재분화에 효과적이었으며 AgNO$_3$는 10-20 $\mu$M농도에서 재분화가 잘되었고 잎 절편도 갈변되지 않고 푸르게 유지되었는데 이것은 AgNO$_3$가 에틸렌 발생을 효과적으로 억제했기 때문으로 생각된다. Sucrose는 3%보다 낮은 농도에서 shoot 유기가 효과적이었고 CuSO$_4$는 애틀란틱품종 감자 잎 절편으로부터 식물체 재분화에 영향을 주지 못하였다.

Keywords

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