Journal of Korean Society for Atmospheric Environment (한국대기환경학회지)

Korean Society for Atmospheric Environment (한국대기환경학회)
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Effects of Airborne Samples Collected in Yeochun on Gap Junctional Inter cellular Communication in WBF-344 Rat Liver Epithelial Cells

여천공단 일부지역의 대기오염물질이 WBF-344간 상피세포의 Gap Junctional Intercellular Communication에 미치는 영향

  • 양재만 (서울대학교 수의과대학 공중보건학실) ;
  • 박재학 (서울대학교 수의과대학 실험동물학실) ;
  • 김윤신 (한양대학교 의과대학 계량의학교실) ;
  • 이영순 (서울대학교 수의과대학 공중보건학실)
  • Published : 1997.06.01
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Abstract

We collected airborne complex mixtures in a industrial area of Yeochun, and examined whether these complex mixtures could affect gap junctional intercellular communication (GJIC) in a cultured WBF-344 rat liver epithelial cells (LEC). Since the reduction of GJIC plays an important role in chemical carcinogenesis, measurement of changes of GJIC is a meaningful method to screen carcinogenicity of these mixtures. High and low volume samples were dissolved in dimethyl sulfoxide (DMSO) and tested. Blank filter extractions were also examined for exclud-ing possible toxicity of filter itself, and TPA (12-O-tetradecanoylphorbol-13-acetate) and DMSO were used as positive and negative control, respectively. When the cells were exposed to samples at concentration below that required to maintain rather than 85% cell viability based on the result of neutral red uptake assay, maximal inhibition of GJIC was observed at 1hr after treatment with both high and low volume samples by scrape-loading dye transfer assay. In fluorescence recovery after photobleaching assay, recovery rates via gap junctions were 33%/min in high volume sample and 62%/min in low volume sample. In together, airborne samples collected in Yeochun inhibited GJIC in a cultured WBF-344 rat LEC. These results suggest airborne samples tested in this experiment may attribute to cause a certain type and degree of cancers in in vivo when exposured for some periods.

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References

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