Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)
- Volume 17 Issue 3
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- Pages.285-292
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- 1997
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- 2287-5824(pISSN)
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- 2287-5832(eISSN)
Transformation of Brassica napus with Acid Phosphatase Gene
Acid Phosphatase 유전자 도입에 의한 유채의 형질 전환
- Lee, Hyo-Shin (College of Agriculture, Kyungpook National University) ;
- Son, Dae-Young (Plant Molecular Biology and Biotechnology, Kyungsang National University) ;
- Jo, Jin-Ki (College of Agriculture, Kyungpook National University)
- Published : 1997.09.30
Abstract
This study was conducted to obtain the transgenic Brnssica napus plants with tobacco Apase gene using the binary vector system of Agrobacteriurn fumefociens. The results obtained were summarized as follows: A repressible acid phosphatase gene of Saccharon~yces cerevisiae, pho105 was used for screening of tobacco Apase cDNA. In order to identify Apase gene in tobacco genome, Southern blot analysis was pcrformed and the Apase gcnc may be present as a single copy, or at most two or three copies, in tobacco genome. To isolate the tobacco Apase gene, tobacco cDNA library was constructed using purifed mRNA from -Pi treated tobacco root and the plaque forming unit of the library was 2.8 x
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