Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Isolation and Degradation Characteristics of 2,4,4l-Trichloro-2l-Hydroxydiphenyl Ether Degrading Bacterium

2,4,4l-Trichloro-2l-Hydroxydiphenyl Ether 분해균의 분리 및 분해특성

  • Han, Nan-Sook (Dept. of Microbiogy, Pusan National University) ;
  • Son, Hong-Joo (Dept. of Microbiogy, Pusan National University) ;
  • Lee, Geon (Dept. of Microbiogy, Pusan National University) ;
  • Lee, Sang-Joon (Dept. of Microbiogy, Pusan National University)
  • 한난숙 (부산대학교 미생물학과) ;
  • 손홍주 (부산대학교 미생물학과) ;
  • 이건 (부산대학교 미생물학과) ;
  • 이상준 (부산대학교 미생물학과)
  • Published : 1997.04.01
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Abstract

The bacterial strains, which utilizes 2,4,4'-trichloro-2'-hydroxydiphenyl ether(TCHDPE) as a sole carbon source, were isolated by selective enrichment culture from soil samples of industrial waste deposits. The bacterium that showed the highestt biodegradation activity was designated as EL-O47R The isolated strain EL-O47R was Identified as the genus Pseudomonas from the results of morphological, cultural, and biochemical tests. The optimum conditions of medium for the growth and the degradation of TCHDPE were TCHDPE 500 ppm, (NH4)2SO4 0.1% as the nitrogen source, initial pH 7.0±0.1, and 37℃, respectively. In this conditions, the regradation rate of TCHDPE was about 97%. Pseudomonas sp. EL-O47R was tested for resistance to several metal compounds and antibiotics. Pseudomonas sp. EL-O47R was moderately grown to Cd(NO3)2, ZnCl2, AgSO4, CuSO4 and HgCl2. This strain was sensitive to rifampicin and kanamycln but resistant to ampicillin, penicillin, tetracyclin and chloramphenlcol. Pseudomonas sp. EL-O47R was grown structurally related com- pounds and potential metabolites of TCHDPE, and has the stability on TCHDPE biodegradation.

Keywords

References

  1. Mar. Pollut. v.8 Diphenyl ether-another marine environmental contamination Addison, R.F.
  2. Manual of Methods for General Bacteriology Gerhardt, P.;R.G.E. Murray;R.N. Costilow;E.W. Nester;W.A. Wood;N.R. Krieg;G.B. Phillips
  3. Appl. Environ. Microbiol. v.44 Biodegradation of 2,4,5-trichlorophenoxyacetic acid by a pure culture of Pseudomonas cepacia Kilbane, J.J.;D.K. Chatterjee;J.S. Karns;S.T. Kellogg;A.M. Chakrabarty
  4. Encyclopedia of chemical technology, 2nd ed. v.15 Kink, R.E.;D.F. Othmer
  5. Bergey's Manual of Systematic Bacteriology Krieg, N.R.;J.G. Holt
  6. Appl. Environ. Microbiol. v.58 Kinetic comparison of seven strains of 2,3 -di-chlorophenoxyacetic acid degrading bacteria Linda, E.G.;J.A. Robinson;D.R. Shelton
  7. Biochemical Tests for Identification of Medical Bacteria MacFaddin, J.F.
  8. Aquat. Toxicol. v.9 Biological half lives of chlorinated diphenyl ethers in rainbow trout(Salmo gairdnerl) Niimi, A.J.
  9. Appl. Environ. Microbiol. v.56 Metabolism of dibenzofuran by Pseudomonas sp. HH27 Perter, F.
  10. Chloroaromatic compounds containing oxygen Rappe, C.
  11. Appl. Environ. Microbiol. v.57 Degradation of 1,2,4-trichloro- and 1,2,4,5-tetrachlorobenzene by Pseudomonas strains Sander P.;R.M. Wittich;P. Fortnagal;H. Wilkes;W. Frank
  12. Agric. Biol. Chem. v.50 Microbial degradation products from biphenyl-related compounds Takase, I.;T. Omori;Y. Minoda
  13. Xenobiotica v.9 Metabolism of chlorodiphenyl ethers and Irgasan DP 300 Tulp, M.T.M.;G. Sundstrom;L.B.J.M. Martron;O. Hutzinger
  14. Science v.197 The questions persist where dioxin created a wasteland Walsh, J.