Environmental Analysis Health and Toxicology
- 제12권3_4호
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- Pages.15-22
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- 1997
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- 2671-9525(eISSN)
어류 Metallothionein의 툭성 및 수질오염 평가를 위한 생물모니터링에의 응용
The Characteristics of Fish Metallothionein and Its Application to the Biomonitoring for the Evaluation of Water Pollution
초록
This experiment was performed to examine the immuno-reactive characteristics of fish metal-binding protein, metallothionein (MT), and gain the practical understandings for the proposed use of fish MT as a biomarker. Liver MT induced by Cd in the silver carp was seperated and purified by gel filtration chromatography and ion exchange chromatography. The immuno-reactivity of fish MT was examined with 3 rabbit antisera. Fish MT showed little reactivity with rabbit anti-rat MT antiserum and a weak reactivity with anti-MT peptide antiserum while showed a strong reactivity with rabbit anti-fish MT antiserum. The time-course change of liver MT in the silver carp, after waterborne exposure to 1 ppm of Cd, was checked by Cd-hem method and established competitive ELISA. In both cases, the induction of liver MT showed a good increasing relationship with the exposure days. The results indicate that the fish MT can be developed as a useful biomonitoring means in the toxicological study and for the evaluation of water pollution.
키워드