한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제10권4호
  • /
  • Pages.300-304
  • /
  • 1997
  • /
  • 1226-3591(pISSN)
  • /
  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지

Expression of Low Temperature Regulated Gene H28 in Solanum tuberosum L.

  • 최경화 (생물공학연구소 식물조직배양 R.U) ;
  • 전재흥 (생물공학연구소 식물조직배양 R.U) ;
  • 김현순 (생물공학연구소 식물조직배양 R.U) ;
  • 정영희 (생물공학연구소 식물조직배양 R.U) ;
  • 정혁 (생물공학연구소 식물조직배양 R.U) ;
  • 양덕춘 (한국인삼연초연구원 유전생리부)
  • 발행 : 1997.12.01
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초록

저온관련 유전자인 H28 유전자를 갖고 있는 Agrobacterium MP90균주를 Desiree와 Atlantie 감자 품종의 잎절편과 공동배양한 후 약 6-8주 후에 항생제가 첨가된 선발배지에서 multiple shoots를 얻을수 있었다. 선발된 형질전환체를 대상으로 PCR 방법을 이용한 NPT II 유전자와 H28를 확인한 결과 내한성유전자가 식물체의 genome에 안정적으로 삽입되었음을 확인하였고 northern blot 확인에 의하여 내한성 유전자가 발현됨을 확인하였다.

Leaf dise explants of Solanum tuberosum cultivar. Desiree and Atlantic, were infected with a Agrobacterium MP90 strain containing chimeric gene construct, consisting of antibiotic and low temperature regulated gene (H28) for transformation. regenerated multiple shoots were selected on a medium containing kanamycin and carbenieillin after exposure to Agrobacterium. Both PCR analysis of NPT Ⅱ, H28 genes and northern blot analysis indicated that the genes coding for the enzyme were successfully integrated into the potato genome and could be expressed in potato plants.

키워드

참고문헌

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