RAPD Loci for Seed Protein and Oil Content in Soybean (Glycine max)

  • / J
  • Published : 1997.09.01

Abstract

Seed protein and oil content is important trait in the soybean. Both seed protein and oil content in this plant species is inherited quantitatively. A 68-plant $F_2$ segregation population derived from a mating between Mercury and PI 467.468 was evaluated with random amplified polymorphic DNA (RAPD) markers to identify QTL related to seed protein and oil content. Marker OPB12 was found to be associated with differences in seed protein content. Four markers, OPA09b, OPM07b, OPC14, and OPN11b had highly significant effects on seed oil content. By interval mapping, the interval between marker OPK3c and OPQ1b on linkage group 13 contained a QTL that explained 25.7% variation for seed oil content.

Keywords

References

  1. Korean J. Breed. (Submitted) Quantitative trait locimapping for soybean seed size using RAPD markers. Chung J.;Specht J. E.
  2. Theor. Appl. Genet. v.83 RFLP analysis of soybean seed protein and oil. Diers B.W.;Keim P.;Fehr W.R.;Shoemaker R.C.
  3. Genomics v.1 Mapmaker: an interactive computer package for constructing primary genetic linakge maps of experimental and natural populations. Lander E.S.;Green P.;Abrahamson J.;Barlow A.;Daly M.J.;Lincoln S.E.;Newburg L.
  4. Genetics v.121 Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Lander E.S.;Botstein D.
  5. Whitehead Institutue Technical Report,. Ed.2. Mapping genes controlling quantitative traits with MAPMAKER/QTL 1.1. Lincoln S E.;Daly M J.;Lander E.
  6. Theor. Appl. Genet. v.93 RFLP loci assoicated with soybean seed protein and oil content across populations and locations. Lee S.H.;Bailey M.;Mian M.A.R.;Carter T.E.;Shipe E.R.;Ashley D.A.;Parrott W.;Hussey R.S.;Boerma H.R.
  7. Theor. Appl. Genet. v.86 Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L.). Mansur L.M.;Lark K.G.;Kross H.;Oliveira A.
  8. Proc. Natl. Acad. Sci. USA v.81 Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Saghai Maroof, M.A.;Soliman K.M.;Jorgenson R.;Allard R.W.
  9. Soybenas: improvement, production, and uses, 2nd edn. Agronomy v.16 World distribution and significance of soybean Smith K.J.;Huyser W.;Wilcox J.R.(ed.)
  10. Proc ⅩⅡ Congr EUCARPIA. Marker-based selection for quantitative traits. Stuber C.W.