Enhanced Effect of Gluten Hydorlysate on Solubility and Bioavailability of Calcium in Rats

글루템 가수분해물에 의한 칼슘의 가용화 및 체내이용성 증진 효과

  • 이연숙 (서울대학교 농업생명과학대학 농가정학과)
  • Published : 1997.02.01


Dietary peptides have recently received attention regarding their beneficial effects on nutrient metabolism since the caseinphosphoptides obtained from casein hydrolysate are generally believed to enhance the intestinal absorption of Ca. The two experiments were conducted to investigate the effects of various hydrolyzed fractions of gluten on Ca bioavailability. The gluten hydrolysate of dietary components was produced by enzymatic hydrolysis of gluten whereas gluten hydrolysate supernationt and its precipiate resulted from centrifugation. In experiment I, the rats were for 4 weeks fed the 4 kinds of diets containing same amount of nitrogen and calories and diffeing only in the forms of nitrogen sources. The diets were gluten (G), gluten hydrolysat(GH), gluten hydrolysate supernatant(GHS) and gluten hydrolysate precipitatie(GHP). Determination was made for the body weight gain, serum Ca concentration, Ca solubility in small intestinal contents, bone weight, length and stength, bone ash and Ca content, and Ca balance, respectively. No significant difference was noticed as regards growth, serum Ca, and bone dimension and Ca content among rat groups. More significant increase was observed with regard to Ca absorption and intestinal solubility in the rats receiving the GH or GHS diet which containe crude gluten peptides, than in those subjected to G or GHP diet. In experiment II, in vitro determination for Ca solubility was made to ascertain the mechanism responsible for the effects of gluten peptides on Ca absorption. The 10mM Ca in potassium phosphate buffer solution(pH 7.0) incubated for 3 hours at 37$^{\circ}C$ by the GHS fraction, was observed to be capable of increasing the Ca solubility at 5-25mg/ml concentration of gluten peptides. These observations suggest that the gluten peptides from gluten hydrolysate may enhance the Ca absorption efficiency by increasing the solubility of Ca in small intestine.



  1. 食品機能 Chiba,H.;Yoshikawa,M.
  2. 機能性食品の 硏究 Arai,S.
  3. J. Chem. Tech Biotechnol v.32 Limited enzymic degradation of protein : A new approach in the industrial application of hydrolysate Alder-Nissen,J.
  4. J. Agric. Food Chem. v.36 Solubility and emulsifying properties of casein and whey proteins modified enzymatically by trypsin Chobert,J.M.;Harb,C.B.;Nicholas,M.G.
  5. Gut v.26 Influence of protein composition and hydrolysis method on intestinal absorption of protein in man Keohane,P.;Grimble,G.K.;Brown,B.;Spiller,R.C.;Silk,D.B.A.
  6. Agr. Biol. Chem. v.36 In vivo formation of phosphopeptide with calcium-binding property in the small intestinal tract of the rat fed on casein Naito,H.;Kawakami;Imamura,T.
  7. Br. J. Nutr. v.43 Phosphopeptides and solble calcium in the small intestine of rats given a casein diet Lee,Y.S.;Noguchi,T.;Naito,H.
  8. Br. J. Nutr. v.49 Intestinal absorption of calcium in rats given diets containing casein or amino acid mixture : the role of casein phosphopeptides Lee,Y.S.;Noguchi,T.;Naito,H.
  9. J. Jap. Soc. Nutr. Food Sci. v.45 Supplemental effect of caseinphophopeptides(CPP) on the calcium balance of growing rats Lee,Y.S.;Park,J.;Naito,H.
  10. Intl. Symp. on functional Foods for Health, Proc. Searches for novel sources of functional foods with special reference to calcium nutrition Naito,H.;Lee,Y.S.
  11. J. Bone Miner Met. v.12 Effect of caseinphophopeptide on absorbability of co-ingested calcium in normal postmenopausal women Heaney,R.P.;Saito,Y.;Orimo,H.
  12. Am. J. Physiol v.245 Rat pancreatic response to intestinal infusion of intact and hydrolyzed protein Green,G.M.;Miyasaka,K.
  13. 한국영양학회지 v.26 대두펩타이드의 소화율이 흰쥐의 혈청 콜레스테롤 농동 미치는 영향 한응수;이형주;손동화
  14. Kungl Lantbrukshogskolans Annaler v.22 A turbidimetric method for the determination of higer polyethylene glycol in biological materials Hyden,S.A.
  15. Agr. Biol. Chem. v.26 Relative nutritional value of whole protein, hydrolyzed protein and free amino acids in man Moriaty,K.J.;Hegarty,J.E.;Fairclough,P.D.;Clark,M.J.;Dawson,A.M.
  16. 한국영양학회지 v.28 단백질과 단백질가수분해물이 침수 속박 스트레스에 의해 유도된 위궤양 흰쥐의 질소대사에 미치는 영향 김창임;이연숙
  17. J. Oarent Enteral nutr. v.13 Effect of whey proteins, their oligopeptide hydrolysates and free amino acid mixtures on growth and nitrogen retention in fed and starved rats Poullian,M.G.;Cezard,J.P.;Roger,L.;Mondy,F.
  18. J. Nutr. v.115 Comparative effects of protein, protein hydrolysate, amino acid diets on nitrogen metabolism of normal, protein-deficient, gastrectomized or hepatectomized rats Yamamoto,S.;Korin,T.;Anzai,M.;Wang,M.F.;Inoue
  19. 48th Ann. Meeting Jap Soc Nurt Food Sci Hukuoka Effects of r-polyglutamate on Ca bioavailability Sato,S.;Nosawa,K.;Tanimoto,K.;Honki,M.