Journal of Food Hygiene and Safety (한국식품위생안전성학회지)
- Volume 12 Issue 1
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- Pages.71-77
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- 1997
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- 1229-1153(pISSN)
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- 2465-9223(eISSN)
Determination of Sulfamethazine Using High Performance Liquid Chromatography and Several Screening Methods
HPLC 및 신속검출방법을 이용한 우유내 Sulfamethazine의 분석에 관한 연구
Abstract
Sulfonamides, a therapeutically important group of antimicrobial drugs, are widely used to treat and prevent the acute systemic and skin infections in dairy cattle. They also pose an economic hazard through inhibition of growth of dairy starter cultures. This study was carried out to compare four screening methods for detection of sulfamethazine in milk and determine the positive milk sample by HPLC method. Sulfamethazine was used to spike at five levels of sulfamethazine. The Lac-Tek test and CharmII test were also consistent better than TTCII test and Delvo SP test in sulfamethazine detection. Analysis probabilities of obtaining a positive response with TTCII test and Delvo SP test assay at 50 ppb sulfamethazine level in milk samples were only 14%, 42% each. Whereas using the Lac-Tek test and CharmII test would have resulted in 100% identification of the five levels. Determination of sufamethazine using the HPLC method in the spiked milk were 10.64, 19.30, 30.76,38.83 and 50.23 ppb, respectively.