Preventive Nutrition and Food Science
- Volume 2 Issue 1
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- Pages.61-65
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- 1997
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- 2287-1098(pISSN)
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- 2287-8602(eISSN)
Subcloning of Nodulin 26 Wild Type(S262) and Phosphorylation Site Mutant(S262D) into the Yeast Expression Vector pYES2
- Cha, Youn-Soo (Dept. of Food and Nutrition, Yosu National Fisheries University)
- Published : 1997.03.01
Abstract
Wild type nodulin 26(nod 26) cDNA(S262) and phodphorylation aite mutant(S262D) were constructed by a yeast expression system using pYES2 plasmids(pTES2-D262 and pTES2-S262D) were sc-reened by restriction mapping with BamHI of KpnI. S262 nod 26 contained a sreine residue at position 262 and S262D nod 26 contained the substitution mutation of serine to aspartic acid residue at position 262 were verified by automated floursent DNA sequencing.