Studies on Embryo Cryopreservation and Twinning by Embryo Transfer of Korean Native Cattle: III. Culture and Freezing of IVF Bisected Embryos

한우 수정란의 동결보존 및 쌍자생산에 관한 연구 III. 이분 체외수정란의 배양과 동결

In vitro fertilization(IVF) derived morula and blastocyst embryos were bisected by a simple method and cultured in vitro without zona pellucida And also bisected embryos were frozen-thawed and cultured in vitro) to evaluate the survival rate. The results obtained were as follows : The average number of grade I or II immature follicular oocytes recovered by slicing method per ovary was 11.9 from 142 ovaries. Following in vitro fertilization, the rates of cleavage and in vitro development to morula and blatocyst were 61.7 and 32.2% respectively. The successful bisection rate of IVE embryos was 67.51%, and the embryos of blastocyst stage were bisected successfully at significantly(P