한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제25권5호
  • /
  • Pages.483-489
  • /
  • 1997
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Streptomyces endus YP-1이 생산하는 항암활성 물질의 분리 및 정제

  • 최성원 (연세대학교 생명공학과, 생물산업소재연구센터) ;
  • 김병찬 (연세대학교 생명공학과, 생물산업소재연구센터) ;
  • 최선진 (연세대학교 생명공학과, 생물산업소재연구센터) ;
  • 김동섭 (밀양산업대 식품과학과) ;
  • 여익현 (풀무원 기술연구소) ;
  • 문순옥 (수원대학교 생명과학부) ;
  • 오두환 (연세대학교 생명공학과, 생물산업소재연구센터)
  • 발행 : 1997.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Sulforhodamine B (SRB) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, RNA dot blot and Northern hybridization analysis were performed to screen microorganisms for the production of anticancer agent. Among microorganisms tested, strain YP-1 was selected for its cytotoxicity and ability to reduce the level of c-myc RNA. Strain YP-1 was identified as Streptomyces endus. The anticancer material produced by Streptomyces endus YP-1 was sequentially purified by solvent extraction, silica gel column chromatograpby, preparative TLC and preparative HPLC. The cancer material identified as azalomycin B by the instrumental analyses such as $^{1}$H-NMR, $^{13}$C-NMR, Mass, IR and UV absorption. It was colorless amorphous powder and its molecular weight was 1025.278. Azalomycin B, produced by Streptomyces endus YP-1, showed anticancer activity against several human cancer cell lines and reduction of c-Myc protein level in Colo320 DM cells which was determined by Western blot analysis.

키워드

참고문헌

  1. Cell v.64 Oncogenes and signal transduction Lewis, C. C.;R. A. Kurt;C. Christopher;D. Brain;G. Andrea;K. Rosanna;S. Stephen
  2. Cancer biology Causes of cancer Ruddon, R. W.
  3. Biochem. J. v.290 Nuclear diacylglycerol is inreased during cell proliferation in vivo Hrvoie, B.;Z. Mirzet;D. Nullin;F. I. Robin
  4. Nature v.312 Inositol triphosphate, a novel second messenger in cellular signal transduction Berridge, M. J.;R. F. Irvine
  5. Nature v.311 Stimulation of 3T 3 cells induces transcription of c-fos proto-oncogene Greenberg, M. E.;E. B. Ziff
  6. Nature v.312 Induction of c-fos gene and protein factors procedes activation of c-myc Muller, R.;R. Bravo;J. Burchkardt;T. Curran
  7. TIPS v.12 Signalling targets for anticancer drug developments Garth, P.
  8. Proc. Natl. Acad. Sci. USA. v.80 Homogeneously staining chromosomal regions amplified copies of an abundantly expressed cellular oncogene (c-myc) in colon carcinoma Kari, A.;S. Manfred;C. C. Lin;E. Harold;J. M. Bishop
  9. Oncogene v.6 Oncogene transformation frequency of nonsenescent SFME cells is increased by c-myc Cathleen, R.;S. Scanetaka;C. Paul;N. Tetsuya;B. David
  10. Anticancer Res v.11 c-fos and c-myc oncoprotein extression in human hepatocellular carcinomas Patrick, A.;K. Michael;F. Wyn
  11. Cancer Res v.47 Evaluation of tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing Carmichael, J.;W. G. DeGraff;A. F. Gazdar;J. D. Minna;J. B. Mitchell
  12. Bergey's Manual of Determinative Bacteriology(9th ED) Holt, J. G.;N. R. Krieg;P. H. A. Sneath;J. T. Staley;S. T. Williams
  13. Bergey's Mannual of Systematic Bacteriology v.4 Williams, S. T.;M. E. Sharpe;J. G. Holt
  14. J. Gen. Microbiol. v.112 Numerical Taxonomy of Actinomadura and Related Actionomycetes Goodfellow, M.;G. Alderson
  15. J. Gen. Microbiol. v.129 Numerical Classification of Streptomyces and Related Genera Williams, S. T.;M. Goodfellow;G. Alderson;E. M. H. Wellington;P. H. A. Sneath;M. J. Sackin. M. J.
  16. J. Gen. Microbiol. v.129 A Probability Matrix for Identification of some Streptomycetes Williams, S. T.;M. Goodfellow;E. M. H. Wellington;J. C. Vickers;G. Alderson;P. H. A. Sneath;M/J. Sackin;A. M. Mortimer
  17. Appl. Microbiol. v.25 Laboratory Identification of Clinically Important Aerobic Actinomycetes Berd, D.
  18. Giorn. Microbiol. v.7 Melanosporin and elaiophylin, new antibiotics from Streptoomyces melanosporus (sive elanosporofaciens)n. sp. Acarmone, F. M.;C. Bertazzoli;M. Ghione;T. Scotti
  19. Japan. J. Pharmacol. v.55 Inhibitory effects of Copiamycin A, a macrocyclic lactone antibiotic, on gastric $H^+$, $K^+$-ATPase, acid secretion and ulcer formation Hamagishi, Y.;K. Kawano;H. Kamei;T. Oki
  20. J. Microbiol. Biotech. v.6 Structure Determination and Bilogical Activities of Elaiophylin Produced by Streptomyces sp. MCY-846 Lee, S. Y.;M. S. Kim;H. S. Kim;Y. H. Kim;S. D. Hong;J. J. Lee