내열성 포도당 이성화효소를 생산하는 Streptomyces chibaensis J-59의 분리 및 동정

  • Published : 1997.02.01

Abstract

A bacterial strain J-59 was isolated from a humus soil, which produced simultaneously a thermostable glucose isomerase as well as xylanase. The morphological, cultural and physiological characteristics of the isoisomerase strain J-59 were detemined by the use of the media and methods described in International Streptomyces Project. The chemotaxonomic characteristics of the isolated strain J-59 were determined by the analysis of G+C molar % of DNA, diaminipimelic acid, composition of fatty acid and menaquinone. As the results of various examinations, the strain J-59 was identified to be Streptomyces chibaensis. This strain produced glucose isomerase intracellularly and xylanase extracellularly when grown in a medium containing xylan, but it was not able to utilize the xylose or xylan as a carbon source. The glucose isomerase of S. chibaensis J59 was highly thermostable, which retained more than 75% activity in the presence of Co$^{2+}$ at 80$\circ $C for 72 h.

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References

  1. Process Biochem. v.15 Glucose isomerase(a review) Chen, W. P.
  2. Agri. Biol. Chem. v.29 Enzymatic conversion of D-glucose to D-fructose. Part II. Properties of the enzyme from Streptomyces phaeochromogenes Tsumura, N.;M. Hagi;T. Sato
  3. Agric. Biol. Chem. v.30 Studies on sugar-isomerizing enzyme production utilization of glucose isomerase from Streptomyces sp Takasaki, Y.
  4. '95 Agricultural Biotechnology Symposium Molecualr determinants of the thermozyme activity and stability: analysis of xylose isomerase and amylopullulanse, Enzyme for Carbohydrate Engineering II Zeikus, J. G.
  5. J. Biol. Chem. v.192 A new spectrophotometric method for the detection and determination at keto sugars and trioses Dische, Z.;E. Borenfreund
  6. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miller, G. L.
  7. Int. J. Syst. Bacteriol. v.16 Methods for characterization of Streptomyces species Shirling, E. B.;D. Gottlieb
  8. Method in Microbiology v.4 Actinomycetes Williams, S. T.;T. Cross
  9. Appl. Microbiol. v.11 System of color wheels for Streptomycete taxonomy Tresner, H. D.;E. J. Backus
  10. J. Gen. Microbiol. v.129 A numerical classification of Streptomyces and related genea Williams, S. T.;M. Goodfellow;G. Alderson;E. M. H. Wellington;P. H. A. Sneath;M. J. Sackin
  11. J. Gen. Microbiol. v.129 A probability matrix for identification of some Streptomycetes Williams, S. T.;M. Goodfellow;G. Alderson;E. M. H. Wellington;P. H. A. Sneath;M.J. Sackin
  12. J. Gen. Appl. Microbiol. v.16 Taxonomic studies on Coryneform Bacteria II. Principal amino acid in the cell wall and their taxonomic significance Yamada, K.;K. Komagata
  13. FEMS Microbiology Lett. v.25 Determination of DNa base composition by reversed-phase high-performance liquid chromatography Tamaoka, J.;K. Komagata
  14. Methods in Microbiology v.19 Lipid and cell wall analysis in bacterial systematics Komagata, T.;K. I. Suzuki;R. R. Colwell(ed.);R. Grigorova(ed.)
  15. J. Appl. Bacteriol. v.54 Analysis of bacterial menaquinone mixtures by high performance liquid chromatography Tamaoka, J.;Y. Katayama;H. Kuraish
  16. Int. J. Syst. Bacteriol. v.18 Cooperative description of type cultures of Streptomyces. II. Speices description from first study Shirling, E. B.;D. Gottleib
  17. Int. J. Syst. Bacteriol. v.18 Cooperative description of type cultures of Streptomyce. III. Additional species description from first and second studies Shirling, E. B.;D. Gottlieb
  18. Int. J. Syst. Bacteriol. v.19 Cooperative description of type culture of Streptomyces. IV. Species descriptions from the second, third and fourth studies Shirling, E. B.;D. Gottleb
  19. Int. J. Syst. Bacteriol. v.22 Cooperative description of type cultures of Streptomyces. V. Additional descriptions Shirling, E. B.;D. Gottlieb
  20. Bergey's Manual of Systemactic Bacteriology (9th) v.4 Streptomycetes and related genera Locci, R.;S. T. Williams(ed.);M. E. Sharpe(ed.);J. G. Holt(ed.)
  21. J. Ferment. Technol. v.52 Key for classification and identification of 458 species of the Streptomycetes included in ISP Nonomura H.
  22. Appl. Microbiol. v.12 Rapid differentiation between Nocardia and Streptomyces by paper chromatograpy of whole cell hydrlysates Becker, B.;M. P. Lechevalier;R. E. Gordon;H. A. Lechevalier
  23. J. Antibiol. v.11 Collocidin, a new antibiotic Suzuki, S.;G. Nakamura;K. Okuma;Y. Tomiyama
  24. Interantional Biodeterioration Bulletin v.17 Role of some mesophilic soil organisms in rice stubble degradation Chtterjee, S. K.;B. Nandi
  25. Genetics and Molecular Biology of Industrial Microorganisms Genetic, enzymatic, and crystallographic studies of the glucose isoerase of two Streptomyces species Tiraby, G.;S. Bejar;D. Drocout;J. P. Reynes;P. J. Sicard;G. K. Farber;A. Glasfeld;D. Ringe;G. A. Petsko;O. L. Hershberger(et al.)(ed.)
  26. Appl. Environ. Microbiol. v.61 xy/A cloning and sequencing and biochemical characterization of xylose isomerase from Thermotoganeapolitana Vielille, C.;J. M. Hess;R. M. Kelly;J. G. Zeikus
  27. Appl. Microl. Biotechnol. v.43 Isolation and characterization of a D-glucose/xylose isomerase from a new thermophilic strain Streptomyces sp. (PLC) Inyang, C. U.;U. Gebhart;S. K. C. Obi;H. Bisswanger