Biotechnology and Bioprocess Engineering:BBE
- 제2권2호
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- Pages.82-85
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- 1997
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- 1226-8372(pISSN)
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- 1976-3816(eISSN)
Gene Expression using nar Promoter under Anaerobic Condition with Recombinant E. coli
- Kim, Kwang-Soo (Division of Environmental and Biological Engineering, Kangwon National University) ;
- Lee, Jong-Won (Daparment of Biochemistry, School of Medicine, Taegu Hyosung Catholic University) ;
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Hong, Eock-Kee
(Division of Environmental and Biological Engineering, Kangwon National University)
- 발행 : 1997.12.01
초록
The nar promoter as an inducible promoter was characterized for the process development for the gene expression and the protein production under anaerobic condition. The LB medium was selected as a main culture medium showing the enzyme activity of 18,000 units/min/g cell in the flask cultivation. The optimum concentration of nitrate was 1%. Under anaerobic conditions, the gene expression was fully induced in the presence of nitrate.