Biotechnology and Bioprocess Engineering:BBE
- Volume 2 Issue 2
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- Pages.82-85
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- 1997
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- 1226-8372(pISSN)
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- 1976-3816(eISSN)
Gene Expression using nar Promoter under Anaerobic Condition with Recombinant E. coli
- Kim, Kwang-Soo (Division of Environmental and Biological Engineering, Kangwon National University) ;
- Lee, Jong-Won (Daparment of Biochemistry, School of Medicine, Taegu Hyosung Catholic University) ;
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Hong, Eock-Kee
(Division of Environmental and Biological Engineering, Kangwon National University)
- Published : 1997.12.01
Abstract
The nar promoter as an inducible promoter was characterized for the process development for the gene expression and the protein production under anaerobic condition. The LB medium was selected as a main culture medium showing the enzyme activity of 18,000 units/min/g cell in the flask cultivation. The optimum concentration of nitrate was 1%. Under anaerobic conditions, the gene expression was fully induced in the presence of nitrate.