Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Mating-type-specific inhibition of phosphorylation by sexual pheromone (Rh. A) on heterobasidiomycetous yeast Rhodosporidium toruloides.

이담자효모 Rhodosporidium toruloides의 성pheromone(Rh.A)에 의한 성접합형 특이적 인산화 저해 반응

  • Published : 1997.12.01
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Abstract

Two phosphorylated proteins having molecular weights of 57kD and 72kD were detected from the slubilized membrane protein fraction of mating type a cells of Rhodosporidium toruloides which belongs to heterobasidiomycetous yeast. The phosphorylation of the protein was inhibited by a sexual pheromone, Rhodotorucine A (Rh. A), which is secreted from mating type a cells. On the other hand, counterpart mating type A cells and M-39 strain which is a styerile mutant derived from a cells, had also the same two phosphorylated proteins, However, the phosphorylation of the protein from A cells, and M-39 strain were not inhibited by the Rh. A. It suggests that inhibition of the phosphorylation reaction by the Rh. A in mating type a cells is a mating-type-specific reaction that relate to transduction mechanism of sexual pheromone signaling.

Keywords

References

  1. J. Gen. Appl. Microbal v.13 Studies on Sexuality of Rhodotorula Bnno, I.
  2. J. Gen. Appl. Microbial v.24 Morphological change in the early stages of Rhodosporidium toruloides M1057, a Strain of mating type a Abe, K.;Ikusaka;S. Fukui
  3. Agric. Biol. Chem. v.43 Structure of rhodotorucine A, a peprydyl factor inducing maating tube formation in Rhodosporidium toruloides Kamija, Y.;A. sakurai;S. Tamura;E. Tsuchiya;K. Abe;S. Fukui
  4. J. Bactriol v.151 Role of metacolism of the mating phermone in sexual differentiation of the heterobasidiomycetous Rodosporidium toruloides Miyakawa, T.;M. Nishihara;E. Tsuchiya;S. Fukui
  5. J. Bactriol. v.162 Involvement of protein sulfhydryls in the reigger reaction of rhodotorucine A, a farnesyl peptide mating pheromone of Rhodosporidium toruloides Miyakawa, T.;Y. K. Jeong;E. Tsuchiya;S. Fukui
  6. J. Bacteriol v.169 Purification and characterization of a $ca^{²+}$ dependent membrane peptidase involced in the signaling of mating pheromone in Rhodosporidium toruloides Miyakawa, T.;Y. K. Jwong;M. Kaji, E. Tsuchiya;S. Fukui
  7. Eur. J. Biochem. v.169 Interaction with phospholipids of a membrane thiol pepridase that is essential for the signal transduction of mating pheromone in Rhodosporidium toruloides Jeoung, Y. K.;T. Miyakawa;A. Imabayashi Tsuchiya;S. fukui
  8. J. Bacteriol. v.162 Transient increase $ca^{²+}$ uptake as signal for malting pheromone-induced differntiation in the herterobasidiomycetous yeast Rhodosporidium toruloides Miyakawa, T. T.;Tachikawa;Y. K. Jeong;E. Tsuchiya;S. Fukui
  9. Kor. J. Appl. Microbiol. Biotechnol. v.24 no.6 Relation of $ca^{²+}$ ATPase and Triggerprptidase(TPase) that are membrane proteins in a differentiation process on heterobasidiomycetous yeast Rhodsoporidium toruroides Jeong, Y. K.;T. H. Lee;K. T. Jeong
  10. Kor. J. Appl. Microbiol. Biotechnol. v.24 no.6 Inhibition of membrane proteins in a differentiation process on hetrobasidiomycetous yeast Rhodosporidium torulodides Jeong, Y. K.;T. H. Lee;B. H. Ryu
  11. Ann. Rev. Biocchem. v.39 Chemical synthesis of pepties and peptides and proteins Magline, A.;R. B. Merrifild
  12. Nature v.227 Cleavage of ctructural proteins during the assembly of the head of bavteriophage T₄ Laemmli, U. K.
  13. J. Biol. Chem. v.193 Protein measurement with folin phenol reagent Lowry, O. H.;N. J. Reasbrough