Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Production of Biosurfactant by Tsukamurella sp. 26A

Tsukamurella sp. 26A에 의한 생물계면활성제의 생산

  • 최경숙 (부산대학교 자연과학대학 미생물학과) ;
  • 김순한 (부산대학교 자연과학대학 미생물학과) ;
  • 정영기 (동의대학교 자연과학대학 미생물학과) ;
  • 장경립 (부산대학교 자연과학대학 미생물학과) ;
  • 이태호 (부산대학교 자연과학대학 미생물학과)
  • Published : 1997.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The strain producing biosurfactant was isolated from soil. The isolated strain was identified as the genus Tsukamurella through its morphological, cultural, physiological, menaquinone type, fatty acid composition characteristics. The highest biosurfactant production by Tsukamurella sp. 26A was observed after 4 days cultivation in the culture medium containing n-hexadecane 7%, $NaNO_{3}$ 0.2%, $K_2HPO_4$ 0.001%, $MgSO_{4}$ center dot $7H_{2}O$ 0.02%, $CaCl_2$ center dot $2H_{2}O$ 0.02%, yeast extract 0.02%(pH 6.8-7.0, 30^{\circ}C.$ The surface and interfacial tension of an aqueous solution reached 30 mNim and 1.5 mNim, respectively. The biosurfactant stabilized oil-in-water emulsion with a variety of hydrocarbons, edible oils and petroleum oils.

토양으로부터 biosurfactant를 생산하는 미생물을 분리하여 Tsukamurella sp. 26A로 동정하였다. Biosurfactant 생산을 위한 최적 배지 조성은 n-hexadecane 7%, $NaNO_{3}$ 0.2%, $K_2HPO_4$ 0.001%, $MgSO_{4}$ center dot $7H_{2}O$ 0.02%, $CaCl_2$ center dot $2H_{2}O$ 0.02%, yeast extract 0.02%(pH 6.8-7.0, 30^{\circ}C.$ )이었으며 배양액의 최저 표면장력과 계면장력은 각각 30mN/m, 1.5mN/m였다. 유화기질로서 hydrocarbon류, edible oil류, 그리고 petroleum oil등에 작용시켰을 경우 비교적 높은 유화활성과 유화 안정도를 나타내었다.

Keywords

References

  1. 미생물과 산업 v.14 김상종
  2. Appl. Environ. Microbiol. v.46 Characterization of two extracellular polysaccharides from marine bacteria Boyle, C.D.;A.E. Reade
  3. Monosaccharide Carbohydrate analysis, a practical approach Chaplin, M.F.;J.F. Kennedy
  4. Biotechnol. Bioeng. v.31 Production of biosurfactant from sugar alcohol and vegetable oils catalyzed by lipase in a nonaqueous medium Chopineau, J.;F.D. MacCaafferty;M. Therisod;A.M. Klibanov
  5. Appl. Environ. Microbiol. v.48 Isolation of a bioemulsifier from Candida lipolytica Cirigliano, M.C.;G.M. Carman
  6. Appl. Environ. Microbiol. v.46 Isolation of a bioemulsifier from Candida lipolytica Cirigliano, M.C.;G.M. Carman
  7. Appl. Environ. Microbiol. v.50 Purification and characterization of liposan, a bioemulsifier from Candida lipolytica Cirigliano, M.C.;G.M. Carman
  8. Appl. Environ. Microbiol. v.47 Production of a biosurfactant from Torulopsis bombicola Copper, D.G.;D.A. Paddock
  9. Annu. Rev. Microbiol. v.31 Oil tankers and pollution: a microbiological approach Gutnick, D.L.;E. Rosenberg
  10. Advan. Biochem. Engineer. Biotechnol. v.33 Extracellular microbial lipids as biosurfactants Haferburg, D.;R. Hommel
  11. Bergey's Manual of Determinative bacteriology (9th ed.) John G. Holt;N.R.Krieg;P.H.A.Sneath;J.T.Staley;S.T.Williams
  12. Bergey's Manual of Determinative bacterio- logy(9th ed.) John G. Holt;N.R. Krieg;P.H.A. Sneath;J.T. Staley;S.T. Williams
  13. Kor. J. Appl. Microbiol. Biotechnol. v.24 Emulsifying agent production by Acinetobacter sp. BE-254 Kim, S.H.;J.D. Lee;B.C. Kim;T.H. Lee
  14. J. Microbiol. Biotechnol. v.6 Purification and characterization of bioemulsifier by Acinetobacter sp. BE-254 Kim, S.H.;J.D. Lee;B.C. Kim;T.H. Lee
  15. Kor. J. Biotechnol. Bioeng. v.7 Effects of culturing parameters on the production of microbial biosurfactant from Candida bombiocola Kim, W.K.;E.K. Kim
  16. Kor. J. Biotechnol. Bioeng v.7 Application of biosurfactant (sophorolipid) produced from Candida bombiocola Kim, W.K.;E.K. Kim
  17. Biotechnology. v.3 Microbial emulsifiers and de-emulsifiers Kosaric, N.;N.C.C. Gray;W.L. Cairn
  18. Biosurfactant and biotechnology Kosaric, N.;W.L. Carins
  19. Appl. Environ. Microbiol. v.44 Chemical and physical characterization of interfacial-active lipids from Rhodococcus erythropolis grown on n-alkanes Kretschmer, A.;H. Bock;F. Wabner
  20. Chem. Eng. News. v.63 Industrial surfactants set for strong growth Layman, P.
  21. Biotechnol. Bioeng. v.21 Production and surface-active properties of microbial surfactants Magaritis, A.;J.E. Zajic;D.F. Gerson
  22. Develop. Ind. Microbiol. v.20 Biosurfactant production by Nocardia erythropolis Margaritis, A.;K. Kennedy;J.E. Jajic;D.F. Gerson
  23. Kor. J. Appl. Microbiol. Bioeng. v.16 Emulsification of bunker-C oil by a marine bacterium Achromobacter sp. M-1220 Park, J.Y.;I.S. Park;K.H. Suh;Y.K. Hong
  24. Biotechnol. Advs. v.3 Biosurfactants Parkinson, M.
  25. J. Bacteriol. v.154 Localization of emulsan-like polymers associated with the cell surface of Acinetobacter calcoaceticus Pines, O.;E.A. Bayer;D.L. Gutnick
  26. Appl. Microbiol. Biotechnol. v.31 Penta- and disaccharide lipid formation by Nocardia corynebacteroids grown on n-alkanes Powalla, M.;S. Lang;V. Wary
  27. Appl. Environ. Microbiol. v.48 Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as carbon source Satos, L.G.p;O. kappeli;A. Fiechter
  28. Biotech. Letters v.18 Synthesis of biodegradable emulsifier using lipase in anhydrous pyridine Sin, Y.M.;S.H. Chung;J.Y. Park;T.H. Lee
  29. Bergey's Manual of Systematic Bacteriology v.4 Standely. T.W.;M.E. Sharpe;J.G. Holt
  30. J. Korean Ind. Eng. Chem. v.5 Studies on physical behavior of alkyl polyglucosides (I) -Interfacial activities and detergency- Yoon, Y.K.;K.S. Choi
  31. CRC Crit. Rev. Microbiol. Nov. Bio-emulsifiers Zajic, J.E.;C.J. Panchal
  32. Biotech. Bioeng. v.26 Properties of hydrocarbon-in-water emulsions stabilized by Acinetobacter RAG-1 Emulsan Zosim, Z.;D. Gutnick;E. Rosenberg